The Dilator Naris Muscle as a Reporter of Facial Nerve Regeneration in a Rat Model.
The Dilator Naris Muscle as a Reporter of Facial Nerve Regeneration in a Rat Model.
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DOI：
10.1097/sap.0000000000000273
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发表时间：
2016-01
影响因子：
1.5
通讯作者：
Hadlock TA
中科院分区：
文献类型：
--
作者：
Weinberg JS;Kleiss IJ;Knox CJ;Heaton JT;Hadlock TA
许多研究人员使用大鼠胡须垫模型研究面神经再生，尽管尚未开发出广泛标准化的啮齿动物面神经再生结果测量方法。产生胡须伸展的内在胡须垫“吊索”肌肉位于每根胡须的底部，非常小，很难整体研究。在这里，我们比较了胡须垫神经支配的两种潜在报告肌肉的功能神经支配：鼻孔扩张器和上唇提肌，以表征面神经再生。通过测量刺激单个面神经分支期间的收缩力和复合肌肉动作电位，以及通过测量扩张器鼻孔远端肌腱释放之前和之后的搅拌幅度，阐明了扩张器鼻孔和提上唇肌的运动供应。鼻孔扩张器的神经支配模式与固有的胡须垫肌肉组织的模式相匹配（即通过面神经的颊侧和下颌边缘分支），而上唇提肌似乎几乎完全由颧支支配，其主要目标是眼轮匝肌。虽然上唇提肌通常被用作胡须垫神经支配的报告肌肉，但目前的数据表明，其神经支配模式与产生胡须伸展的肌肉基本上没有重叠。扩张鼻孔肌可能作为胡须垫神经支配的更合适的报告者，因为它由与内在胡须垫肌肉组织相同的面部神经分支支配，使得结构/功能相关性更准确，并且与研究面部神经再生的研究者更相关。
Many investigators study facial nerve regeneration using the rat whisker pad model, though widely standardized outcomes measures of facial nerve regeneration in the rodent have not yet been developed. The intrinsic whisker pad “sling” muscles producing whisker protraction, situated at the base of each individual whisker, are extremely small and difficult to study en bloc. Here, we compare the functional innervation of two potential reporter muscles for whisker pad innervation: the dilator naris and the levator labii superioris, to characterize facial nerve regeneration. Motor supply of the dilator naris and levator labii superioris was elucidated by measuring contraction force and compound muscle action potentials during stimulation of individual facial nerve branches, and by measuring whisking amplitude before and after dilator naris distal tendon release. The pattern of dilator naris innervation matched that of the intrinsic whisker pad musculature (i.e. via the buccal and marginal mandibular branches of the facial nerve), whereas the levator labii superioris appeared to be innervated almost entirely by the zygomatic branch, whose primary target is the orbicularis oculi muscle. While the levator labii superioris has been commonly used as a reporter muscle of whisker pad innervation, the present data show that its innervation pattern does not overlap substantially with the muscles producing whisker protraction. The dilator naris muscle may serve as a more appropriate reporter for whisker pad innervation because it is innervated by the same facial nerve branches as the intrinsic whisker pad musculature, making structure\function correlations more accurate, and more relevant to investigators studying facial nerve regeneration.