Understanding the role of serological and clinical data on assessing the dynamic of malaria transmission: a case study of Bagamoyo district, Tanzania.
Understanding the role of serological and clinical data on assessing the dynamic of malaria transmission: a case study of Bagamoyo district, Tanzania.
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DOI：
10.11604/pamj.2022.43.60.35779
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发表时间：
2022
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据报告，自然获得的血液阶段疟疾抗体和疟疾临床数据有助于监测疟疾随时间的变化，并可作为疟疾感染的标志。本研究评估了Bagamoyo市5-17个月婴儿恶性疟原虫抗体的总免疫球蛋白G（IgG）水平，利用常规卫生设施监测数据估计疟疾发病率，并预测了抗疟原虫抗体与疟疾发病率之间的趋势关系。252份血清样本用于通过酶联免疫吸附试验评估总IgG，结果以任意单位（Au）表示。252份样本中有147份是在2021年的血液阶段疟疾疫苗试验中采集的[ClinicalTrials.gov NCT 04318002]，105份是2012年进行的疟疾疫苗试验的存档样本[ClinicalTrials.gov NCT 00866619]。疟疾发病率是根据2013年至2020年期间从District-Health-Information-Software-2（DHIS 2）检索的疟疾快速检测或血涂片阳性结果的门诊数据计算的。使用STATA第14版分析了两项研究的横断面数据。该研究表明，总抗-IgG水平从2012年的490.21 AU下降到2021年的97.07 AU，这与发病率从2013年的58.25例/1000人年下降到2020年的14.28例/1000人年有关。我们还观察到，在按性别比较疟疾高传播区和低传播区时，发病率存在显著差异。然而，我们没有观察到性别和研究年份之间的总抗抗体差异。总抗疟原虫抗体水平似乎是评估疟疾流行地区婴儿疟疾传播动态的一个重要血清学暴露标志。
naturally acquired blood-stage malaria antibodies and malaria clinical data have been reported to be useful in monitoring malaria change over time and as a marker of malaria exposure. This study assessed the total immunoglobulin G (IgG) levels to Plasmodium falciparum schizont among infants (5-17 months), estimated malaria incidence using routine health facility-based surveillance data and predicted trend relation between anti-schizont antibodies and malaria incidence in Bagamoyo. 252 serum samples were used for assessment of total IgG by enzyme-linked immunosorbent assay and results were expressed in arbitrary units (AU). 147/252 samples were collected in 2021 during a blood-stage malaria vaccine trial [ClinicalTrials.gov NCT04318002], and 105/252 were archived samples of malaria vaccine trial conducted in 2012 [ClinicalTrials.gov NCT00866619]. Malaria incidence was calculated from outpatient clinic data of malaria rapid test or blood smear positive results retrieved from District-Health-Information-Software-2 (DHIS2) between 2013 and 2020. Cross-sectional data from both studies were analysed using STATA version 14. this study demonstrated a decline in total anti-schizont IgG levels from 490.21AU in 2012 to 97.07AU in 2021 which was related to a fall in incidence from 58.25 cases/1000 person-year in 2013 to 14.28 cases/1000 person-year in 2020. We also observed a significant difference in incidence when comparing high and low malaria transmission areas and by gender. However, we did not observe differences when comparing total anti-schizont antibodies by gender and study year. total anti-schizont antibody levels appear to be an important serological marker of exposure for assessing the dynamic of malaria transmission in infants living in malaria-endemic regions.