Orbital fluid shear stress promotes osteoblast metabolism, proliferation and alkaline phosphates activity in vitro
Orbital fluid shear stress promotes osteoblast metabolism, proliferation and alkaline phosphates activity in vitro
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DOI：
10.1016/j.yexcr.2015.07.002
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发表时间：
2015-09-10
影响因子：
3.7
通讯作者：
Froemming, G. R. A.
中科院分区：
文献类型：
--
作者：
Aisha, M. D.;Nor-Ashikin, M. N. K.;Froemming, G. R. A.
肌肉骨骼系统的长期废用与机械负荷减少和缺乏合成代谢刺激有关。作为一种力学信号，多向眼眶流体切应力将合成代谢信号传递给成骨细胞，促进细胞分化、代谢和增殖。信号通过细胞骨架框架传递，直接修饰基因和蛋白质表达。因此，我们的目的是研究正常人成骨细胞（NHOst）细胞骨架的组织与眼眶流体剪切（OFS）应力。特别感兴趣的是NHOst代谢，增殖和成骨功能标志物的细胞骨架重组的后果。在振荡培养箱中以250 RPM刺激细胞，72 h后导致肌动蛋白和微管蛋白纤维重排。轨道切应力增加NHOst线粒体代谢和增殖，同时防止细胞凋亡。RANKL/OPG比值降低，表明眼眶切应力有可能抑制破骨细胞生成和破骨细胞活性。ALP活性和OCN蛋白产生的增加表明刺激保留了成骨细胞功能。可能通过肌动蛋白产生的剪切应力似乎保持成骨细胞代谢和功能标志物的合成代谢反应增强。我们假设，通过施加适当大小和持续时间的眼眶剪切应力作为非药物合成代谢治疗，可以帮助改善长期废用病例的骨再生。(C)2015 Elsevier Inc. All rights reserved.
Prolonged disuse of the musculoskeletal system is associated with reduced mechanical loading and lack of anabolic stimulus. As a form of mechanical signal, the multidirectional orbital fluid shear stress transmits anabolic signal to bone forming cells in promoting cell differentiation, metabolism and proliferation. Signals are channeled through the cytoskeleton framework, directly modifying gene and protein expression. For that reason, we aimed to study the organization of Normal Human Osteoblast (NHOst) cytoskeleton with regards to orbital fluid shear (OFS) stress. Of special interest were the consequences of cytoskeletal reorganization on NHOst metabolism, proliferation, and osteogenic functional markers. Cells stimulated at 250 RPM in a shaking incubator resulted in the rearrangement of actin and tubulin fibers after 72 h. Orbital shear stress increased NHOst mitochondrial metabolism and proliferation, simultaneously preventing apoptosis. The ratio of RANKL/OPG was reduced, suggesting that orbital shear stress has the potential to inhibit osteoclastogenesis and osteoclast activity. Increase in ALP activity and OCN protein production suggests that stimulation retained osteoblast function. Shear stress possibly generated through actin seemed to hold an anabolic response as osteoblast metabolism and functional markers were enhanced. We hypothesize that by applying orbital shear stress with suitable magnitude and duration as a non-drug anabolic treatment can help improve bone regeneration in prolonged disuse cases. (C) 2015 Elsevier Inc. All rights reserved.