Colorimetric detection of mercury, lead and copper ions simultaneously using protein-functionalized gold nanoparticles
Colorimetric detection of mercury, lead and copper ions simultaneously using protein-functionalized gold nanoparticles
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使用蛋白质功能化金纳米颗粒同时比色检测汞、铅和铜离子
DOI：
10.1016/j.bios.2011.03.033
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发表时间：
2011-06-15
影响因子：
12.6
通讯作者：
Jiang, Xingyu
中科院分区：
文献类型：
--
作者：
Guo, Yongming;Wang, Zhuo;Jiang, Xingyu
开发了一种使用木瓜蛋白酶功能化的金纳米颗粒（P-AuNPs）检测Hg 2+、Pb 2+和Cu 2+的简单、经济和快速的比色方法。木瓜蛋白酶是一种具有7个半胱氨酸残基的蛋白质，它可以选择性地与Hg 2+、Pb 2+和Cu 2+结合。我们用木瓜蛋白酶功能化了金纳米颗粒。P-AuNPs可用于同时检测Hg 2+、Pb 2+和Cu 2+，并基于金纳米颗粒聚集诱导的颜色变化对水溶液中的3种离子表现出不同的响应。P-AuNPs对水中汞离子的响应最明显，而对铅和铜离子的响应最弱，真实的水样分析验证了这一结论。P-AuNPs溶液的pH值、P-AuNPs的浓度和金纳米颗粒的尺寸对检测系统的灵敏度有影响，并且我们发现较大的金纳米颗粒有助于更灵敏的结果。该检测系统可以使用42 nm的金纳米颗粒检测低至200 nM的Hg 2+、Pb 2+或Cu 2+。我们希望我们的方法在发展中地区有广泛的应用，用于监测某些地区的水质。(C)2011爱思唯尔有限公司版权所有。
A simple, cost-effective and rapid colorimetric method for any or all of Hg2+ Pb2+ and Cu2+ detection using papain-functionalized gold nanoparticles (P-AuNPs) has been developed. Papain is a protein with seven cystein residues, which can selectively bind with Hg2+, Pb2+ and Cu2+. We functionalized gold nanoparticles with papain. The P-AuNPs could be used to simultaneously detect Hg2+, Pb2+ and Cu2+, and showed different responses to the three ions in an aqueous solution based on the aggregation-induced color change of gold nanoparticles. The P-AuNPs displayed the most obvious response to mercury ions in water in contrast to lead and copper ions, and the real water sample analysis verified the conclusion. The sensitivity of the detection system was influenced by the pH of the P-AuNPs solution, the concentration of P-AuNPs and the size of gold nanoparticles, and we found that larger gold nanoparticles contributed to more sensitive results. The detection system can detect as low as 200 nM Hg2+, Pb2+ or Cu2+ using 42 nm gold nanoparticles. We expect our approach to have wide-ranging applications in the developing region for monitoring water quality in some areas. (C) 2011 Elsevier B.V. All rights reserved.