Cross-talk between sumoylation and phosphorylation in mouse spermatocytes.
Cross-talk between sumoylation and phosphorylation in mouse spermatocytes.
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DOI：
10.1016/j.bbrc.2017.04.107
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发表时间：
2017-06-03
影响因子：
3.1
通讯作者：
Vigodner M
中科院分区：
文献类型：
--
作者：
Xiao Y;Lucas B;Molcho E;Vigodner M
减数分裂的G2/M1过渡主要受到翻译后的修饰，但在不同的sumoylation中，不同的变化后，在包括多种发育过程中，在包括多种元素的情况下，在多个发育过程中出现了相关的sumoylation。 Sumoylation在男性繁殖中的作用仅具有Begun to be elucidated. Given the important role of several SUMO targets (including kinases) in meiosis, in this study, the role of sumoylation was addressed by monitoring the G2/M1 transition in pachytene spermatocytes in vitro upon inhibition of sumoylation. Furthermore, to better understand the cross-talk between sumoylation and phosphorylation, the activity of several kinases implemented in meiotic progression was also assessed upon down-regulation of sumoylation.分析的结果表明，用牙龈酸（GA）抑制sum酰基（GA）在小鼠的精子中阻止了染色体凝结和脱离染色体，我们的结果表明，在G2/M1 Meiotiot thribition中， C-ABL，ERK和AKT的活动不是GA治疗后受影响或增加。
The meiotic G2/M1 transition is mostly regulated by posttranslational modifications, however, the cross-talk between different posttranslational modifications is not well-understood, especially in spermatocytes. Sumoylation has emerged as a critical regulatory event in several developmental processes, including reproduction. In mouse oocytes, inhibition of sumoylation caused various meiotic defects and led to aneuploidy. However, the role of sumoylation in male reproduction has only begun to be elucidated. Given the important role of several SUMO targets (including kinases) in meiosis, in this study, the role of sumoylation was addressed by monitoring the G2/M1 transition in pachytene spermatocytes in vitro upon inhibition of sumoylation. Furthermore, to better understand the cross-talk between sumoylation and phosphorylation, the activity of several kinases implicated in meiotic progression was also assessed upon down-regulation of sumoylation. The results of the analysis demonstrate that inhibition of sumoylation with ginkgolic acid (GA) arrests the G2/M1 transition in mouse spermatocytes preventing chromosome condensation and disassembling of the synaptonemal complex. Our results revealed that the activity of PLK1 and the Aurora kinases increased during the G2/M1 meiotic transition, but was negatively regulated by the inhibition of sumoylation. In the same experiment, the activity of c-Abl, the ERKs, and AKT were not affected or increased after GA treatment. Both the AURKs and PLK1 appear to be “at the right place, at the right time” to at least, in part, explain the meiotic arrest obtained in the spermatocyte culture.