Aging impairs regulation of ryanodine receptors from extensor digitorum longus but not soleus muscles.
Aging impairs regulation of ryanodine receptors from extensor digitorum longus but not soleus muscles.
复制标题
衰老会损害趾长伸肌而非比目鱼肌对兰尼碱受体的调节。
DOI：
10.1002/mus.26063
复制
发表时间：
2018
期刊：
影响因子：
3.4
通讯作者：
Balog,EdwardM
中科院分区：
文献类型：
--
作者：
Gaboardi,AngelaJ;Kressler,Jochen;Snow,TeresaK;Balog,EdwardM
导读：由于兴奋-收缩耦合受损和肌浆网（SR） Ca2+释放减少可能导致骨骼肌力量的年龄相关下降，我们研究了衰老对生理通道配体对ryanodine受体（RyR1）骨骼肌异构体的调节作用。方法：[3H]Ryanodine结合8月龄和26月龄Fischer 344指长伸肌（EDL）和比目鱼肌膜，研究年龄对Ca2+和calmodulin （CaM）调节RyR1的影响。结果：衰老降低了Ca2+刺激的EDL膜的最大结合。在0.3 μM Ca2+中，年龄降低了与EDL膜的结合，CaM增加了与EDL膜的结合。在300 μM Ca2+中，CaM降低了结合，但年龄效应不显著。衰老不影响比目鱼RyR1的Ca2+或CaM调节。讨论：在衰老的快缩肌中，RyR1 Ca2+调节受损可能导致SR Ca2+释放降低和肌肉功能降低。中国生物医学工程学报（英文版），2018
Introduction: Because impaired excitation‐contraction coupling and reduced sarcoplasmic reticulum (SR) Ca2+release may contribute to the age‐associated decline in skeletal muscle strength, we investigated the effect of aging on regulation of the skeletal muscle isoform of the ryanodine receptor (RyR1) by physiological channel ligands.Methods: [3H]Ryanodine binding to membranes from 8‐ and 26‐month‐old Fischer 344 extensor digitorum longus (EDL) and soleus muscles was used to investigate the effects of age on RyR1 modulation by Ca2+and calmodulin (CaM).Results: Aging reduced maximal Ca2+‐stimulated binding to EDL membranes. In 0.3 μM Ca2+, age reduced binding and CaM increased binding to EDL membranes. In 300 μM Ca2+, CaM reduced binding, but the age effect was not significant. Aging did not affect Ca2+or CaM regulation of soleus RyR1.Discussion: In aged fast‐twitch muscle, impaired RyR1 Ca2+regulation may contribute to lower SR Ca2+release and reduced muscle function.Muscle Nerve57: 1022–1025, 2018