The A component (SmhA) of a tripartite pore-forming toxin from Serratia marcescens: expression, purification and crystallographic analysis.
The A component (SmhA) of a tripartite pore-forming toxin from Serratia marcescens: expression, purification and crystallographic analysis.
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DOI：
10.1107/s2053230x20013862
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发表时间：
2020-12-01
期刊：
影响因子：
--
通讯作者：
Baker PJ
中科院分区：
文献类型：
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作者：
Churchill-Angus AM;Sedelnikova SE;Schofield THB;Baker PJ
SmhA是机会性人致病菌粘质沙雷菌（Serratia marcescens）中三组分α-成孔毒素的A组分，已被克隆、过表达和纯化。对硒代蛋氨酸衍生蛋白进行了晶体生长，收集了异常数据，计算了相，并绘制了初始电子密度图。三方α-成孔毒素由三种蛋白质（A， B和C）构成，存在于许多细菌病原体中。虽然来自革兰氏阴性菌的B和C组分的结构已经被描述，但革兰氏阴性α-成孔毒素的A组分的结构迄今为止被证明是难以捉摸的。SmhA是机会性人致病菌粘质沙雷菌的A组分，已被克隆、过表达和纯化。培养了硒代蛋氨酸衍生蛋白晶体，并收集了异常数据。计算了相，得到了初始电子密度图。
SmhA, the A component of the tripartite α-pore-forming toxin from the opportunistic human pathogen Serratia marcescens, has been cloned, overexpressed and purified. Crystals were grown of selenomethionine-derivatized protein, anomalous data were collected, phases were calculated and an initial electron-density map was produced. Tripartite α-pore-forming toxins are constructed of three proteins (A, B and C) and are found in many bacterial pathogens. While structures of the B and C components from Gram-negative bacteria have been described, the structure of the A component of a Gram-negative α-pore-forming toxin has so far proved elusive. SmhA, the A component from the opportunistic human pathogen Serratia marcescens, has been cloned, overexpressed and purified. Crystals were grown of selenomethionine-derivatized protein and anomalous data were collected. Phases were calculated and an initial electron-density map was produced.