Bovine beta-casein micelles as delivery systems for hydrophobic flavonoids
Bovine beta-casein micelles as delivery systems for hydrophobic flavonoids
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DOI：
10.1016/j.foodhyd.2019.06.005
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发表时间：
2019-11-01
影响因子：
10.7
通讯作者：
Kleijn, J. Mieke
中科院分区：
文献类型：
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作者：
Li, Mo;Fokkink, Remco;Kleijn, J. Mieke
牛奶蛋白β-酪蛋白（β-CN）是一种本质上非结构化的两亲性蛋白质，可自组装成胶束。柚皮素是葡萄柚中主要的疏水性黄烷酮，具有几种有益的生物学作用：例如，它表现出抗氧化、抗癌和抗炎活性。本文表明，柚皮素可以封装在β-CN胶束。采用荧光光谱、分子对接模型、动态光散射（DLS）、静态光散射（SLS）和等温滴定量热法（ITC）研究了柚皮素对蛋白质缔合行为和胶束性质的影响。柚皮素在pH 7和pH 2下均与β-氯化萘结合，促进形成具有明确尺寸分布的胶束并稳定胶束。结果发现，与纯β-CN胶束相比，含柚皮素的β-CN胶束具有较低的临界胶束浓度（CMC）和较大的聚集数（N-agg）。SLS和多角度DLS结果表明，纯β-CN胶束和含柚皮素的β-CN胶束的结构之间存在相当大的差异。在存在柚皮素的情况下，形成具有相对松散的核心的球形胶束（“空心球”），而纯β-CN胶束较小，似乎是椭圆形的。值得注意的是，通过在胶束中摄取柚皮素，可以显著提高水溶液中柚皮素的浓度。这些发现导致的结论是，β-CN胶束是非常有前途的有效的疏水性生物活性化合物的纳米载体。
The milk protein beta-casein (beta-CN) is an intrinsically unstructured amphiphilic protein that self-assembles into micelles. Naringenin is the main hydrophobic flavanone in grapefruit and has several beneficial biological effects: it exhibits, for example, antioxidant, anticancer and anti-inflammatory activity. This paper shows that naringenin can be encapsulated in beta-CN micelles. Fluorescence spectroscopy, molecular docking modelling, dynamic light scattering (DLS), static light scattering (SLS) and isothermal titration calorimetry (ITC) were applied to characterize the effect of naringenin on the protein association behavior and properties of the resulting micelles. Naringenin binds to beta-CN at both pH 7 and pH 2, promotes the formation of micelles with a well-defined size distribution and stabilizes the micelles. It was found that naringenin-containing beta-CN micelles have a lower critical micelle concentration (CMC) and a larger aggregation number (N-agg) compared to pure beta-CN micelles. SLS and multi-angle DLS results suggest considerable differences between the structures of pure beta-CN micelles and naringenin-containing beta-CN micelles. In the presence of naringenin spherical micelles were formed with a relatively loose core ("hollow sphere"), while the pure beta-CN micelles are smaller and seem to be elliptic. Notably, by uptake of naringenin in the micelles, the concentration of naringenin in aqueous solution could be raised considerably. These findings lead to the conclusion that beta-CN micelles are very promising as effective delivery nano-vehicles for hydrophobic bioactive compounds.