Expression of Programmed Death-Ligand 1 in Laryngeal Carcinoma and its Effects on Immune Cell Subgroup Infiltration
Expression of Programmed Death-Ligand 1 in Laryngeal Carcinoma and its Effects on Immune Cell Subgroup Infiltration
复制标题
程序性死亡配体1在喉癌中的表达及其对免疫细胞亚群浸润的影响
DOI：
10.1007/s12253-018-0501-x
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发表时间：
2019-10-01
影响因子：
2.8
通讯作者：
Xu, Chengbi
中科院分区：
文献类型：
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作者：
Yu, Dan;Cheng, Jinzhang;Xu, Chengbi
目的研究人喉鳞状细胞癌组织中程序性死亡配体1（PD-L1）的表达及其对CD 8+肿瘤浸润淋巴细胞（TIL）和肿瘤相关巨噬细胞（TAM）的影响。2010年1月至2015年12月，69例喉癌患者和10例声带白斑患者在吉林大学第二附属医院（吉林长春）颈外科接受肿瘤切除术。采用免疫组化法检测PD-L1、CD 8、CD 16、CD 206在喉癌、癌旁组织及声带白斑组织中的表达。分析PD-L1表达与临床病理特征、TAM表达及CD 8 +T细胞浸润的关系。PD-L1在喉癌组织中的表达显著高于癌旁组织和白斑组织。PD-L1的表达与喉癌的临床分期、组织分化程度及颈淋巴结转移密切相关。PD-L1表达与CD 8 + TIL和CD 16+细胞（M1型TAM）数量呈负相关，而与CD 206+（M2型TAM）呈正相关。PD-L1在喉癌组织中高表达，肿瘤微环境免疫抑制。
To study the expression of programmed death-ligand 1 (PD-L1), and its effects on CD8+tumor infiltrating lymphocytes (TILs) and tumor associated macrophages (TAMs) in human laryngeal squamous cell carcinoma. Sixty-nine patients with laryngeal carcinoma and 10 with vocal cord leukoplakia received tumor resection at Neck Surgery Department in the Second Affiliation Hospital of Jilin University (Changchun, Jilin) from Jan. 2010 to Dec. 2015. The expressions of PD-L1, CD8, CD16 and CD206 in laryngeal carcinoma, paracancerous and vocal cord leukoplakia tissues were detected with immunohistochemistry. The associations between PD-L1 expression and clinicopathologic features, expression of TAMs and CD8+T cell infiltration were analyzed. Expression of PD-L1 is significantly higher in laryngeal carcinoma than in paracancerous or leukoplakia tissue. The expression of PD-L1 is closely associated with stage of laryngeal cancer, histological differentiation and neck lymphatic metastasis. PD-L1 expression is negatively correlated with the number of CD8+TILs and CD16+cells (M1 type TAMs), while is positively associated with CD206+(M2 type TAMs). PD-L1 is highly expressed in the laryngeal cancer with the tumor microenvironment immunosuppression.