Buffer Standards for the Biological pH of the Amino Acid N-[2 hydroxyethyl]piperazine-N'-[3-propanesulfonic acid], HEPPS, From (278.15 to 328.15) K.
Buffer Standards for the Biological pH of the Amino Acid N-[2 hydroxyethyl]piperazine-N'-[3-propanesulfonic acid], HEPPS, From (278.15 to 328.15) K.
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氨基酸 N-[2 羟乙基]哌嗪-N-[3-丙磺酸] 的生物 pH 缓冲标准品，HEPPS，从 (278.15 至 328.15) K。
DOI：
10.1021/je200629y
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发表时间：
2011
影响因子：
--
通讯作者：
Henson,IsaacB
中科院分区：
文献类型：
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作者：
Roy,LakshmiN;Roy,RabindraN;Wollen,JoshuaT;Harmon,MeaganA;Stegner,JessicaM;Shah,AnkitaA;Henson,IsaacB
对于所研究的HEPPS缓冲液，有7种不含NaCl的缓冲液和8种含cl的缓冲液，它们的离子强度（I= 0.16 mol·kg-1）与血浆相似。使用扩展的debye - h<s:1> ckel方程和Bates-Guggenheim惯例，在（278.15至328.15）K的温度范围内对这些缓冲溶液进行了评估。先前确定的dej值已用于确定HEPPS缓冲溶液在（298.15和310.15）k下的工作pH值，这些建议作为在等渗离子强度ofI= 0.16 mol·kg-1的盐水介质中pH测量的二级标准参考溶液。
For the HEPPS buffer under investigation, there are seven buffer solutions without NaCl and eight buffer solutions that contain Cl–and have an ionic strength (I= 0.16 mol·kg–1) that is similar to that of blood plasma. These buffer solutions have been evaluated in the temperature range of (278.15 to 328.15) K using the extended Debye–Hückel equation and the Bates–Guggenheim convention. The previously determinedEjvalues have been used to determine the operational pH values of HEPPS buffer solutions at (298.15 and 310.15) K. These are recommended as secondary standard reference solutions for pH measurements in saline media with an isotonic ionic strength ofI= 0.16 mol·kg–1.