A quantitative study of MC3T3-E1 cell adhesion, morphology and biomechanics on chitosan-collagen blend films at single cell level
A quantitative study of MC3T3-E1 cell adhesion, morphology and biomechanics on chitosan-collagen blend films at single cell level
复制标题
单细胞水平壳聚糖-胶原蛋白共混膜上 MC3T3-E1 细胞粘附、形态和生物力学的定量研究
DOI：
10.1016/j.colsurfb.2015.04.037
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发表时间：
2015-08-01
影响因子：
5.8
通讯作者：
Zhou, Chang-ren
中科院分区：
文献类型：
--
作者：
Wang, Chuang;Xie, Xu-dong;Zhou, Chang-ren
在组织工程中，细胞与生物材料之间的相互作用对细胞的增殖和分化起着关键作用。然而，对这些相互作用的定量分析却没有得到很好的研究。本研究的目的是定量地概括MC 3 T3-E1细胞在壳聚糖-胶原膜上的粘附、形态学和生物力学性能的差异。本文采用原子力显微镜-单细胞力谱（AFM-SCFS）技术，对MC 3 T3-E1细胞与一系列壳聚糖-胶原膜之间的解结合力进行了实时、原位的研究。同时，用原子力显微镜检测了不同壳聚糖-胶原膜上细胞形态和杨氏模量的变化。细胞面积和CCK-8结果显示，细胞伸展和增殖随着胶原含量的增加而增加。AFM观察清楚地显示细胞高度降低，伪足融合，胶原含量增加。细胞粘附力从0.76 +/- 0.17 nN增加至1.70 +/- 0.19 nN。相反，反映细胞生物物理变化的细胞杨氏模量分别从11.94 +/- 3.19 kPa降至1.81 +/- 0.52 kPa。这表明更强的细胞-基质相互作用有利于细胞粘附，更好的细胞柔性有利于细胞铺展。研究结果表明，细胞形态，粘附力和杨氏模量显着影响各种壳聚糖胶原基板。这些方法和定量结果对研究壳聚糖和/或胶原基细胞靶向药物载体的作用机理以及壳聚糖-胶原复合生物材料的制备具有指导意义。(C)2015 Elsevier B. V.版权所有。
The interaction between cells and biomaterials plays a key role in cell proliferation and differentiation in tissue engineering. However, a quantitative analysis of those interactions has been less well studied. The objective of this study was to quantitative recapitulate the difference of MC3T3-E1 cell adhesion, morphological and biomechanical properties on chitosan-collagen films in terms of chemical composition. Here, the unbinding force between MC3T3-E1 cell and a series of chitosan-collagen films was probed by a real-time and in situ atomic force microscopy-single cell force spectroscopy (AFM-SCFS). Meanwhile, changes in cell morphology and Young's modulus on different chitosan-collagen films were detected by AFM. The cell area and CCK-8 results showed that cell spreading and proliferation increased with increasing collagen content. AFM observations clearly showed cell height decreased and pseudopod fusion with the collagen content increased. Cell adhesive force increased from 0.76 +/- 0.17 nN to 1.70 +/- 0.19 nN. On the contrary, cells Young's modulus, which reflected biophysical changes of cells decreased from 11.94 +/- 3.19 kPa to 1.81 +/- 0.52 kPa, respectively. It suggested that stronger cell-substrate interactions benefit cell adhesion, and better cell flexibility improve cell spreading. The findings indicate that cell morphology, adhesive force and Young's modulus are significant affected by various chitosan-collagen substrates. Those methods and quantitative results have guiding significance for investigating the mechanism of chitosan and/or collagen based cell-targeting drug carrier and the preparation of chitosan-collagen composite biomaterials. (C) 2015 Elsevier B.V. All rights reserved.