ATYPICAL GUANYLYL CYCLASE FROM THE POND SNAIL LYMNAEA STAGNALIS: CLONING, SEQUENCE ANALYSIS AND CHARACTERIZATION OF EXPRESSION
ATYPICAL GUANYLYL CYCLASE FROM THE POND SNAIL LYMNAEA STAGNALIS: CLONING, SEQUENCE ANALYSIS AND CHARACTERIZATION OF EXPRESSION
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DOI：
10.1016/j.neuroscience.2009.11.008
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发表时间：
2010-02-03
期刊：
影响因子：
3.3
通讯作者：
Korneev, S. A.
中科院分区：
文献类型：
--
作者：
Ribeiro, M.;Schofield, M.;Korneev, S. A.
可溶性胍基环化酶（sGCs）传统上被认为是一氧化氮（NO）的主要分子受体，一氧化氮是一种参与神经系统许多功能的气态递质。然而，一些sGCs对NO不敏感，因此被称为非典型。尽管非典型sGCs已被证明存在于脊椎动物和无脊椎动物的神经系统中，但我们对它们的功能作用的理解是不完整的。本文报道了一种名为Lym-sGC β 3的非典型sGC的克隆、测序和定位。我们发现Lym-sGC β 3与一些先前表征的非典型sgc具有许多结构特征，包括在调控域中存在Tyr140。这种残基被认为在确定非典型sgc对氧的敏感性方面具有至关重要的意义。这些发现提高了Lym-sGC β 3是一种氧受体的可能性。我们的原位杂交和RT-PCR实验结果进一步支持了这一观点，表明Lym-sGC β 3在osphradium中表达，osphradium是一种外周感觉器官，其中有氧感神经元。同样有趣的是，我们观察到淋巴中枢神经系统中的许多神经元共同表达常规和非典型sGC亚基。这些数据与非典型sGC亚基可能通过形成具有低酶活性的异源二聚体而发挥主要的负调节作用一致。(c) 2010 ibro。Elsevier Ltd.出版。版权所有。
Soluble guanylyl cyclases (sGCs) are traditionally recognized as the main molecular receptor for nitric oxide (NO), a gaseous transmitter involved in many functions of the nervous system. Some sGCs are however insensitive to NO and therefore are known as atypical. Although atypical sGCs have been shown to exist in both vertebrate and invertebrate nervous systems, our understanding of their functional role is incomplete. Here we report on the cloning, sequencing and localization of an atypical sGC named Lym-sGC beta 3 from the snail Lymnaea stagnalis. We found that Lym-sGC beta 3 shares a number of structural characteristics with some previously characterized atypical sGCs including the presence of Tyr140 in the regulatory domain. This residue is thought to be of a critical importance in determining sensitivity of atypical sGCs to oxygen. These findings raise the possibility that Lym-sGC beta 3 is an oxygen receptor. The results of our in situ hybridization and RT-PCR experiments support this idea further by showing that Lym-sGC beta 3 is expressed in the osphradium, a peripheral sense organ in which oxygen-sensing neurons are located. Also of interest are our observations that many neurons in Lymnaea CNS co-express conventional and atypical sGC subunits. These data are consistent with a possible dominant negative regulatory role of atypical sGC subunits through the formation of heterodimers exhibiting low enzymatic activity. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved.