Asymmetric division of cyst stem cells in Drosophila testis is ensured by anaphase spindle repositioning
Asymmetric division of cyst stem cells in Drosophila testis is ensured by anaphase spindle repositioning
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DOI：
10.1242/dev.057901
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发表时间：
2011-03-01
期刊：
影响因子：
4.6
通讯作者：
Hunt, Alan J.
中科院分区：
文献类型：
--
作者：
Cheng, Jun;Tiyaboonchai, Amita;Hunt, Alan J.
许多干细胞不对称分裂以平衡自我更新和分化。在果蝇睾丸中，两个干细胞群体，生殖系干细胞（GSC）和体细胞囊肿干细胞（CySC），相互凝聚和调节。在这里，我们报告说，CySCs分裂不对称，通过重新定位有丝分裂纺锤体周围后期。CySC纺锤体的重新定位需要功能性中心体、动力蛋白和肌动蛋白-膜接头Moesin。需要后期纺锤体重新定位以实现CySC中的高保真不对称分裂，从而保持GSC和CySC数量。我们建议动态主轴重新定位允许CySC不对称地分裂，同时容纳它们封装的GSC的结构。
Many stem cells divide asymmetrically to balance self-renewal and differentiation. In Drosophila testes, two stem cell populations, germline stem cells (GSCs) and somatic cyst stem cells (CySCs), cohere and regulate one another. Here, we report that CySCs divide asymmetrically through repositioning the mitotic spindle around anaphase. CySC spindle repositioning requires functional centrosomes, Dynein and the actin-membrane linker Moesin. Anaphase spindle repositioning is required to achieve high-fidelity asymmetric divisions in CySCs, thus maintaining both GSC and CySC numbers. We propose that dynamic spindle repositioning allows CySCs to divide asymmetrically while accommodating the structure of the GSCs they encapsulate.