Differential expression of ST6GAL1 in the tumor progression of colorectal cancer
Differential expression of ST6GAL1 in the tumor progression of colorectal cancer
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ST6GAL1在结直肠癌肿瘤进展中的差异表达
DOI：
10.1016/j.bbrc.2017.03.167
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发表时间：
2017
影响因子：
3.1
通讯作者：
Zhang Yan
中科院分区：
文献类型：
--
作者：
Zhang Sen;Lu Jishun;Xu Zhijue;Zou Xia;Sun Xue;Xu Yingjiao;Shan Aidong;Lu Jiaoyang;Yan Xialin;Cui Yalu;Yan Wei;Du Yuguo;Gu Jianguo;Zheng Minima;Feng Bo;Zhang Yan
β-半乳糖苷α2，6-唾液酸基转移酶1(ST6GAL1)在结直肠癌中表达升高，在结直肠癌的发生发展中起重要作用。在这里，我们发现在62对配对的肿瘤/正常组织中，ST6GAL1在非转移性肿瘤(I期和II期)中的表达显著高于在转移性肿瘤(III期和IV期)中的表达。为了阐明ST6GAL1如何影响结直肠癌进展的分子机制，我们在结肠腺癌细胞系SW480中进行了ST6GAL1底物的全球鉴定。通过代谢标记和蛋白质组学分析，共鉴定出318个受ST6GAL1过表达影响的膜蛋白。随后的生物信息学分析揭示了一系列可能调节ST6GAL1在结直肠癌中不同功能的潜在底物，包括细胞运动、细胞死亡和存活。综上所述，这些结果表明ST6GAL1在结直肠癌进展过程中表达的动态变化，并提供了与ST6GAL1在结直肠癌中不同功能相关的唾液酸化蛋白列表。
Elevated expression of β-galactoside α2,6-sialyltranferase 1 (ST6GAL1) has been observed in colorectal cancer (CRC) and demonstrated to be important for its tumorigenesis. Here, we found that ST6GAL1 expression was significantly higher in non-metastatic tumors (stage I and II) than that in metastatic tumors (stage III and IV) using 62 pair-matched tumor/normal tissues. To elucidate the molecular mechanisms of how ST6GAL1 affected the CRC progression, we performed a global identification of the substrates of ST6GAL1 in the colon adenocarcinoma cell line SW480. A total of 318 membrane proteins were identified differentially affected by ST6GAL1 overexpression using metabolic labeling and proteomic analysis. Subsequent bioinformatic analysis revealed a list of potential substrates that might mediate the different functions of ST6GAL1 in CRC including cell movement, cell death and survival. Taken together, these results indicate a dynamic change in the expression of ST6GAL1 during the CRC progression and provide a list of sialylated proteins potentially relevant to the different functions of ST6GAL1 in CRC.