Antibody to Langerin/CD207 localizes large numbers of CD8α+ dendritic cells to the marginal zone of mouse spleen
Antibody to Langerin/CD207 localizes large numbers of CD8α+ dendritic cells to the marginal zone of mouse spleen
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DOI：
10.1073/pnas.0812247106
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发表时间：
2009-02-03
影响因子：
11.1
通讯作者：
Steinman, Ralph M.
中科院分区：
文献类型：
--
作者：
Idoyaga, Juliana;Suda, Nao;Steinman, Ralph M.
树突状细胞（DC）在策略上定位以吸收抗原并启动适应性免疫。一个DC子集在小鼠中表达CD8 Alphaα，专门用于捕获垂死的细胞和MHC I类“交叉出现”的抗原。由于CD8（+）DC还表达位于脾T细胞区域的DEC205/CD205，因此认为CD8（+）DC也仅限于T区域。在这里，我们使用了一种与Langerin/CD207的新抗体，该抗体将其分离为CD8（+）CD205（+）DCS，用于免疫标记脾脏。 MAB标记具有高水平CD11C和CD8的离散细胞。出乎意料的是，大多数CD207（+）曲线都位于周围脾脏白色果肉结节周围的边缘区域中，在T细胞区域中只有较小的数量，其中CD205 collabeling被发现。尽管处于边缘区域的位置，但CD207（+）DC缺乏鉴定出3种不同类型的巨噬细胞的分子，该分子位于接近性中，与巨噬细胞相反，边缘区DC是对可溶性和颗粒物底物的较差的清除剂。用微生物激动剂刺激后，兰格蛋白的表达在6-12小时从边缘区消失，但在T细胞区域大大扩展，到24-48小时，Langerin表达消失了。因此，抗链林抗体将大多数CD8（+）DC定位于小鼠脾脏的非T细胞区域，它们与相邻的巨噬细胞不同。
Dendritic cells (DCs) are strategically positioned to take up antigens and initiate adaptive immunity. One DC subset expresses CD8 alpha alpha in mice and is specialized to capture dying cells and process antigens for MHC class I "cross-presentation." Because CD8(+) DCs also express DEC205/CD205, which is localized to splenic T cell regions, it is thought that CD8(+) DCs also are restricted to T zones. Here, we used a new antibody to Langerin/CD207, which colabels isolated CD8(+) CD205(+) DCs, to immunolabel spleen sections. The mAb labeled discrete cells with high levels of CD11c and CD8. Surprisingly most CD207(+) profiles were in marginal zones surrounding splenic white pulp nodules, and only smaller numbers were in T cell areas, where CD205 colabeling was noted. Despite a marginal zone location, CD207(+) DCs lacked identifying molecules for 3 different types of macrophages, localized in proximity and, in contrast to macrophages, marginal zone DCs were poor scavengers of soluble and particulate substrates. After stimulation with microbial agonists, Langerin expression disappeared from the marginal zone at 6-12 h, but was greatly expanded in the T cell areas, and by 24-48 h, Langerin expression disappeared. Therefore, anti-Langerin antibodies localize a majority of CD8(+) DCs to non-T cell regions of mouse spleen, where they are distinct from adjacent macrophages.