Dissolved organic phosphorus use by the invasive freshwater diazotroph cyanobacterium, Cylindrospermopsis raciborskii
Dissolved organic phosphorus use by the invasive freshwater diazotroph cyanobacterium, Cylindrospermopsis raciborskii
复制标题
入侵性淡水固氮蓝藻 Cylindrospermopsis raciborskii 对溶解有机磷的利用
DOI：
10.1016/j.hal.2014.06.015
复制
发表时间：
2014-10-01
期刊：
影响因子：
6.6
通讯作者：
Wu, Zhongxing
中科院分区：
文献类型：
--
作者：
Bai, Fang;Liu, Rui;Wu, Zhongxing
本研究研究了重氮型蓝藻Cylindropermopsis raciborskii对不同溶解有机磷(DOP)化合物的生理响应，以探讨这种入侵物种的环境适应机制。结果表明，在含有β-甘油磷酸、D-葡萄糖-6-磷酸和(2-氨基乙基)-膦酸的条件下，C rasiborskii细胞的比生长率显著高于无磷培养的细胞。我们观察到，细胞最大净光合作用在D-葡萄糖-6-磷酸培养时最高，而在草甘膦培养时最低。类似地，在D-葡萄糖-6-磷酸处理的培养基中，观察到光合作用活性的速率(最大量子效率、最大电子传递速率和光合作用效率)最高。我们报道，碱性磷酸酶对不同有机磷的活性比率随着溶解的无机磷(DIP)浓度的变化而显著变化；这一结果得到了ELF(R)97磷酸盐染料(ELFP)揭示的绿色荧光产物的数量和碱性磷酸酶(PhoA)基因上调的支持。我们的结果表明，在磷有限的情况下，环斑扇贝能够利用不同的有机磷来支持其生长。此外，我们还发现C raciborskii对磷酸盐(C-O-P)的利用率高于磷酸盐(C-P)。这些结果表明，对环境磷的战略灵活性可能在罗氏沼虾的优势地位中起着重要作用。(C)2014爱思唯尔B.V.保留所有权利。
This study examines the physiological responses of the diazotrophic cyanobacteria, Cylindrospermopsis raciborskii, to different dissolved organic phosphorus (DOP) compounds to explore mechanisms of environmental acclimation in this invasive species. Our results show that the specific growth rates of C raciborskii cells in media treated with beta-glycerol phosphate, D-glucose-6-phosphate, and (2-aminoethyl)-phosphinic acid were significantly higher than those of cells grown in phosphorus free media. We observed that maximal net photosynthesis was highest when cells were cultured with D-glucose-6-phosphate and lowest when cells were cultured with glyphosate. Similarly, rates of photosynthetic activity (maximum quantum yield, maximum electron transport rate, and photosynthetic efficiency) were observed to be highest in media treated with D-glucose-6-phosphate. We report that rates of alkaline phosphatase activity to the different organophosphates tested changed markedly in response to the concentration of dissolved inorganic phosphorus (DIP); a result supported by the amount of green fluorescent products revealed by ELF (R) 97 phosphate dye (ELFP) and gene up-regulation for alkaline phosphatase (phoA). Our results indicate that C. raciborskii is able to use different organic phosphorus to support its growth when phosphorus is limited. In addition, we show that C raciborskii has a higher availability to phosphate (C-O-P) than phosphonate (C-P). The results suggest that the strategic flexibility to environmental phosphorus might play an important role in the domination of C raciborskii. (C) 2014 Elsevier B.V. All rights reserved.