DIFFUSION NUCLEAR-MAGNETIC-RESONANCE IMAGING IN EXPERIMENTAL STROKE - CORRELATION WITH CEREBRAL METABOLITES
DIFFUSION NUCLEAR-MAGNETIC-RESONANCE IMAGING IN EXPERIMENTAL STROKE - CORRELATION WITH CEREBRAL METABOLITES
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DOI：
10.1161/01.str.25.2.494
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发表时间：
1994-02-01
期刊：
影响因子：
8.3
通讯作者：
HOSSMANN, KA
中科院分区：
文献类型：
--
作者：
BACK, T;HOEHNBERLAGE, M;HOSSMANN, KA
背景和目的弥散加权核磁共振成像已被证明可以检测实验性卒中中缺血相关的早期改变。这就提出了一个问题，即观察到的信号强度增加是否与脑代谢的变化有关。大脑中动脉闭塞后，记录核磁共振弥散图像，并与脑代谢物的区域浓度和相同平面的组织学进行比较。方法7只麻醉Fischer大鼠，永久性阻断大脑中动脉。7小时后，在3 - 5个平面上测量T-1、T-2和扩散图像（B因子范围为0 - 1500 s/mm（2））。此后，将大脑原位冷冻用于组织学和ATP、葡萄糖、乳酸的定量生物发光成像以及用于组织pH的荧光成像。表观弥散系数从615+/-97x10（-6）.mm（2）.s（-1）（对侧脑）降至359+/-42x10（-6）.mm（2）.s（-1）（缺血性脑;平均值+/-SD，P
Background and Purpose Diffusion-weighted nuclear magnetic resonance imaging has been shown to detect early ischemia-related alterations in experimental stroke. This raises the question of whether the observed increase in signal intensity is correlated with changes in cerebral metabolism. After middle cerebral artery occlusion, nuclear magnetic resonance diffusion images were recorded and compared with the regional concentration of cerebral metabolites and with histology of identical planes. Methods Seven anesthetized Fischer rats were subjected to permanent occlusion of the middle cerebral artery. T-1, T-2, and diffusion images (b factors ranging from 0 to 1500 s/mm(2)) were measured in three to five planes after 7 hours. Thereafter, brains were frozen in situ for histology and quantitative bioluminescence imaging of ATP, glucose, lactate, and for fluorescence imaging of tissue pH. Results Seven hours after middle cerebral artery occlusion, the apparent diffusion coefficient was reduced from 615+/-97x10(-6).mm(2).s(-1) (contralateral brain) to 359+/-42x 10(-6).mm(2).s(-1) (ischemic brain; mean+/-SD, P