Chemical factors of the leaf surface involved in the morphogenesis of Blumeria graminis
Chemical factors of the leaf surface involved in the morphogenesis of Blumeria graminis
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DOI：
10.1006/pmpp.2002.0376
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发表时间：
2002-02
影响因子：
2.7
通讯作者：
M. Tsuba;C. Katagiri;Y. Takeuchi;Y. Takada;N. Yamaoka
中科院分区：
文献类型：
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作者：
M. Tsuba;C. Katagiri;Y. Takeuchi;Y. Takada;N. Yamaoka
大麦（Hordium vulgare）叶片表皮表面和叶蜡诱导禾蓝菌（Blumeria graminis f. sp. hordei）附着胞分化。另一方面，非寄主植物的表皮表面和蜡质在该真菌中诱导的APP分化较少。对大麦蜡的薄层色谱（TLC）组分进行生物测定，结果表明乙醇组分的APP促分化活性最高。气相液相色谱分析表明，大麦醛主要为C26链长醛。在TLC斑点上，小麦黑穗病非寄主植物白菜蜡中的醛类含量高于大麦蜡中的醛类含量。然而，白菜蜡中主要的醛是C28和C30链长。在烟草蜡中，尽管主要醛是与大麦醛一样具有C26链长的醛，但在TLC斑点上的醛含量要少得多，而烟草蜡也是禾草芽孢杆菌的非寄主植物。化学合成的C26醛对禾本科小麦的APP分化具有较强的诱导作用。综上所述，大麦蜡中的C26醛是谷草芽孢杆菌APP分化的重要因子。
The epidermal surface and the wax of leaves of barley (Hordium vulgare) induced appressorium (APP) differentiation of the fungus of Blumeria graminis f. sp. hordei. The epidermal surfaces and waxes of non-host plants, on the other hand, induced less APP differentiation in this fungus. Bioassays of thin-layer chromatographic (TLC) fractions of barley wax revealed that the highest APP differentiation-stimulating activity was in the aldehyde fraction. Gas liquid chromatographic analysis of barley aldehydes showed that the main aldehyde was that with a C26 chain length. The amount of aldehydes in wax of cabbage, a non-host plant of B. graminis, was greater than that in wax of barley in TLC spots. However, the main aldehydes in cabbage wax were those with C28 and C30 chain lengths. In the wax from tobacco, also a non-host plant of B. graminis, the amount of aldehyde in TLC spots was much less, although the main aldehyde was that with a C26 chain length as was found in barley aldehydes. Chemically synthesized C26 aldehyde showed the potential to strongly induce APP differentiation of B. graminis. Therefore, it was concluded that C26 aldehyde, a component of barley wax, is the factor highly responsible for the APP differentiation of B. graminis.