Molecular and antigenic similarities of the fimbrial major components between Porphyromonas gulae and P-gingivalis
Molecular and antigenic similarities of the fimbrial major components between Porphyromonas gulae and P-gingivalis
复制标题
DOI：
10.1016/j.vetmic.2007.09.014
复制
发表时间：
2008-04-01
影响因子：
3.3
通讯作者：
Umemoto, Toshio
中科院分区：
文献类型：
--
作者：
Hamada, Nobushiro;Takahashi, Yusuke;Umemoto, Toshio
古氏卟啉单胞菌是一种与犬牙周炎相关的黑色厌氧菌。关于伴生动物中有色厌氧菌的具体识别和相对发生情况，现有的信息很少。我们的目的是弄清口腔中微生物的黏附和定植所涉及的因素。从古氏拟青霉ATCC 51700菌株中分离纯化了菌毛蛋白。经SDS-PAGE分析，该蛋白的相对分子质量约为41 kDa。抗牙龈假单胞菌ATCC 33277菌毛蛋白的抗体可与古氏肺孢子虫ATCC 51700菌毛蛋白结合。免疫金电镜示41 kDa菌毛抗血清可结合到古雷51700细胞表面的菌毛上。因此，古氏肺孢子虫ATCC 51700菌毛蛋白的大小和抗原性与牙周疫霉ATCC 33277菌毛的大小和抗原性相同。古氏杆菌atcc 51700株的fimA基因与牙龈假单胞菌atcc 33277株的fimA基因核苷酸同源性为94%。此外，推导的氨基酸序列有96.8%的同源性。P-guae对牙龈上皮细胞具有黏附能力。古氏假单胞菌菌毛的性质与牙龈假单胞菌菌毛相似。我们认为，P-guae的表面结构可能在该菌在伴生动物牙周袋中的定植中起作用。(C)2007 Elsevier B.V.保留所有权利。
Porphyromonas gulae is black-pigmented anaerobic bacteria associated with canine periodontitis. There is little information available about the specific identify and relative occurrence of pigmented anaerobes in companion animals. Our aim was to clarify the factor involved in the adherence and colonization of the organism in the oral cavity. Fimbrial protein was purified from P. gulae ATCC 51700. The molecular mass of this protein was approximately 41 kDa as estimated by SDS-PAGE. An antibody against 41-kDa fimbrial protein from P. gingivalis ATCC 33277 reacted with fimbrillin of P. gulae ATCC 51700. Immunogold electron microscopy revealed that the anti-41 kDa fimbrial serum bound to fimbria on the cell surface of R gulae ATCC 51700. Thus, fimbrial protein of P. gulae ATCC 51700 had the same size and antigenicity as 41-kDa fimbriae of P. gingivalis ATCC 33277. The nucleotide sequence of the fimA gene from R gulae ATCC 51700 showed 94% homology with that of P gingivalis ATCC 33277. Moreover, the deduced amino acid sequences have 96.8% identity. P gulae has adherent ability to gingival epithelial cells. The properties of P. gulae fimbriae are similar to those of P gingivalis fimbriae. We suggest that the surface structure of P gulae may play a role in the colonization of this organism in periodontal pockets in companion animals. (c) 2007 Elsevier B.V. All rights reserved.