The implications of stochastic synthesis for the conjugation of functional groups to nanoparticles
The implications of stochastic synthesis for the conjugation of functional groups to nanoparticles
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DOI：
10.1021/bc8002106
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发表时间：
2008-09-01
影响因子：
4.7
通讯作者：
Holl, Mark M. Banaszak
中科院分区：
文献类型：
--
作者：
Mullen, Douglas G.;Desai, Ankur M.;Holl, Mark M. Banaszak
与纳米粒子偶联的配体的随机合成导致每个纳米粒子具有不同数量的配体的群体分布。使用 HPLC 解析和量化了该分布，并且与通过 1 H-1 NMR、凝胶渗透色谱 (GPC) 和电位滴定测量的配体/纳米粒子平均值非常一致，但其分散性明显高于通常认为的单分散性。采用两种统计模型来确认观察到的异质性与理论预期一致。
Stochastic synthesis of a ligand coupled to a nanoparticle results in a distribution of populations with different numbers of ligands per nanoparticle. This distribution was resolved and quantified using HPLC and is in excellent agreement with the ligand/nanoparticle average measured by H-1 NMR, gel permeation chromatography (GPC), and potentiometric titration, and yet significantly more disperse than commonly held perceptions of monodispersity. Two statistical models were employed to confirm that the observed heterogeneity is consistent with theoretical expectations.