Not nanocarbon but dispersant induced abnormality in lysosome in macrophages in vivo
Not nanocarbon but dispersant induced abnormality in lysosome in macrophages in vivo
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DOI：
10.1088/0957-4484/26/19/195102
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发表时间：
2015-05
期刊：
影响因子：
3.5
通讯作者：
M. Yudasaka;Minfang Zhang;S. Matsumura;R. Yuge;T. Ichihashi;H. Irie;K. Shiba;S. Iijima
中科院分区：
文献类型：
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作者：
M. Yudasaka;Minfang Zhang;S. Matsumura;R. Yuge;T. Ichihashi;H. Irie;K. Shiba;S. Iijima
由于用分散剂（通常是磷脂聚乙二醇）涂覆纳米碳，纳米碳的性质从疏水性变为亲水性，用于生物学研究。研究表明，当分散剂注射到小鼠体内时，它们仍然附着在纳米碳上，并影响纳米碳在体内的生物分布。我们在这份报告中表明，分散剂的作用也出现在体内亚细胞水平。碳纳米角（CNH）是一种纳米碳，用神经酰胺聚乙二醇（CPEG）分散并静脉注射到小鼠体内。组织学观察和电子显微镜与能量色散X射线分析表明，在肝脏和脾脏中，溶酶体膜受损，纳米角与巨噬细胞溶酶体中的含铁血黄素形成复合物。推测CPEG分解产生的鞘氨醇损伤溶酶体膜，改变溶酶体内条件，诱导CPEG-CNH和含铁血黄素复合物的形成。作为比较，当使用葡萄糖代替 CPEG 时，既没有发现纳米角-含铁血黄素复合物，也没有发现溶酶体膜损伤。我们的结果表明，表面功能化可以控制纳米碳在体内细胞中的行为，从而提高其医疗应用的适用性。
The properties of nanocarbons change from hydrophobic to hydrophilic as a result of coating them with dispersants, typically phospholipid polyethylene glycols, for biological studies. It has been shown that the dispersants remain attached to the nanocarbons when they are injected in mice and influence the nanocarbons’ biodistribution in vivo. We show in this report that the effects of dispersants also appear at the subcellular level in vivo. Carbon nanohorns (CNHs), a type of nanocarbon, were dispersed with ceramide polyethylene glycol (CPEG) and intravenously injected in mice. Histological observations and electron microscopy with energy dispersive x-ray analysis revealed that, in liver and spleen, the lysosome membranes were damaged, and the nanohorns formed a complex with hemosiderin in the lysosomes of the macrophages. It is inferred that the lysosomal membrane was damaged by sphigosine generated as a result of CPEG decomposition, which changed the intra lysosomal conditions, inducing the formation of the CPEG-CNH and hemosiderin complex. For comparison, when glucose was used instead of CPEG, neither the nanohorn–hemosiderin complex nor lysosomal membrane damage was found. Our results suggest that surface functionalization can control the behavior of nancarbons in cells in vivo and thereby improve their suitability for medical applications.