Anticancer activity and mechanism of Scutellaria barbata extract on human lung cancer cell line A549
Anticancer activity and mechanism of Scutellaria barbata extract on human lung cancer cell line A549
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DOI：
10.1016/j.lfs.2004.05.015
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发表时间：
2004-09-17
期刊：
影响因子：
6.1
通讯作者：
Zhang, Y
中科院分区：
文献类型：
--
作者：
Yin, XL;Zhou, JB;Zhang, Y
半枝莲是原产于中国南部的一种传统中草药，在中国被广泛用于抗炎和利尿。一些研究表明，半枝莲提取物对一些人类癌症具有生长抑制作用。报道了半枝莲提取物治疗肺癌、消化系统癌、肝癌、乳腺癌和绒毛上皮癌的疗效。然而，其抗肿瘤活性的机制尚不清楚。本研究利用人肺癌细胞株A549，对半枝莲的生长抑制作用进行了研究，并对其抗肿瘤作用机制进行了初步探讨。结果表明，半枝莲乙醇提取物对A549细胞生长有明显的抑制作用，其IC50为0.21 mg/ml，其主要作用机制为细胞凋亡和细胞毒作用。基因芯片分析显示，涉及DNA损伤、细胞周期调控、核酸结合和蛋白质磷酸化的16个基因发生了5倍以上的变化。这些数据表明，这些过程与半枝莲介导的对癌细胞的杀伤有关。一个令人惊讶的发现是，与树突状细胞(DC)功能相关的CD209显著下调102倍。需要进一步的功能研究来评估阵列识别的基因在半枝莲介导的抗癌活性中的作用。(C)2004 Elsevier Inc.保留所有权利。
Scutellaria barbata (S. barbata), a traditional Chinese herbal medicine native to southern China, is widely used as an anti-inflammatory and a diuretic in China. Several studies have indicated that extracts of S. barbata have growth inhibitory effects on a number of human cancers. Treatment of lung cancer, digestive system cancers, hepatoma, breast cancer, and chorioepithelioma by S. barbata extracts was reported. However, the mechanism underlying the antitumor activity was unclear. In this study, we studied the growth inhibitory effect of S. barbata and determined its mechanism of antitumor activity using human lung cancer cell line A549. Our results showed that ethanol extracts of S. barbata greatly inhibited A549 cell growth, with IC50 of 0.21 mg/ml. The major mechanisms of inhibition included cell apoptosis and cytotoxic effects. cDNA microarray analysis showed that 16 genes, involved in DNA damage, cell cycle control, nucleic acid binding and protein phosphorylation, underwent more than 5-fold change. These data indicated that these processes are involved in S. barbata-mediated killing of cancer cells. A surprising finding is that CD209, related to dendritic cell (DC) function, was dramatically downregulated by 102-fold. Further functional studies are needed to assess the role of the array-identified genes in S. barbata mediated anticancer activity. (C) 2004 Elsevier Inc. All rights reserved.