Single-cell RNA sequencing data analysis suggests the cell-cell interaction patterns of the pituitary-kidney axis.
Single-cell RNA sequencing data analysis suggests the cell-cell interaction patterns of the pituitary-kidney axis.
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单细胞RNA测序数据分析表明垂体-肾轴的细胞-细胞相互作用模式。
DOI：
10.1038/s41598-022-14680-2
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发表时间：
2022-07-01
影响因子：
4.6
通讯作者：
Zha, Yan
中科院分区：
文献类型：
--
作者：
Deng, Yiyao;Da, Jingjing;Yu, Jiali;Zhou, Chaomin;Yuan, Jing;Zha, Yan
肾功能，包括电解质和水的重吸收和分泌，可能会受到循环激素的影响。脑垂体产生多种激素和细胞因子;然而，这些因素对肾脏的影响尚未得到很好的解释和探索。为了提供更深入的信息和见解来支持垂体-肾轴连接，我们使用来自GEO数据库的小鼠垂体和肾单细胞转录组学数据进行进一步分析。基于配体-受体对分析，垂体和肾细胞类型之间的细胞-细胞相互作用模式进行了描述。关键的配体-受体对，如GH-GHR、PTN-SDC 2、PTN-SDC 4和DLK 1-NOTCH 3，在垂体-肾轴中相对活跃。这些配体-受体对主要靶向近曲小管细胞、主细胞、Henle袢、闰细胞、周细胞、系膜细胞和成纤维细胞，并且这些细胞与生理过程相关，例如物质重吸收、血管生成和组织修复。我们的结果表明，脑垂体可能通过分泌多种激素或细胞因子直接调节肾功能，并表明上述配体-受体对可能代表肾功能或肾脏疾病研究的新研究热点。
Kidney functions, including electrolyte and water reabsorption and secretion, could be influenced by circulating hormones. The pituitary gland produces a variety of hormones and cytokines; however, the influence of these factors on the kidney has not been well explained and explored. To provide more in-depth information and insights to support the pituitary–kidney axis connection, we used mouse pituitary and kidney single-cell transcriptomics data from the GEO database for further analysis. Based on a ligand–receptor pair analysis, cell–cell interaction patterns between the pituitary and kidney cell types were described. Key ligand–receptor pairs, such as GH-GHR, PTN-SDC2, PTN-SDC4, and DLK1-NOTCH3, were relatively active in the pituitary–kidney axis. These ligand–receptor pairs mainly target proximal tubule cells, principal cells, the loop of Henle, intercalated cells, pericytes, mesangial cells, and fibroblasts, and these cells are related to physiological processes, such as substance reabsorption, angiogenesis, and tissue repair. Our results suggested that the pituitary gland might directly regulate kidney function by secreting multiple hormones or cytokines and indicated that the above ligand–receptor pairs might represent a new research focus for studies on kidney function or kidney disease.
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生长激素在健康和肾脏疾病中的肾作用。
DOI：
10.1007/s00467-021-05097-6
发表时间：
2021-08
期刊：
Pediatric nephrology (Berlin, Germany)
影响因子：
--
作者：
Haffner D;Grund A;Leifheit-Nestler M
通讯作者：
Leifheit-Nestler M
影响因子：
48
作者：
Korsunsky, Ilya;Millard, Nghia;Raychaudhuri, Soumya
通讯作者：
Raychaudhuri, Soumya
影响因子：
41.5
作者：
Drube, Jens;Wan, Mandy;Marks, S.
通讯作者：
Marks, S.
影响因子：
4.8
作者：
CHIN, E;ZHOU, J;BONDY, CA
通讯作者：
BONDY, CA
影响因子：
13.6
作者：
Scarpellini, Alessandra;Huang, Linghong;Verderio, Elisabetta A. M.
通讯作者：
Verderio, Elisabetta A. M.