The effect of storage medium tonicity on osteochondral autograft plug diameter.
The effect of storage medium tonicity on osteochondral autograft plug diameter.
复制标题
储存介质张力对骨软骨自体移植物栓直径的影响。
DOI：
10.1016/j.arthro.2010.07.005
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Miniaci,Anthony
中科院分区：
文献类型：
--
作者：
Fening,StephenD;Mihnovets,Jonathon;Jones,MorganH;Midura,RonaldJ;Miniaci,Anthony
本研究的目的是探讨不同的储存介质对骨软骨栓直径的影响。方法评价了四种储存条件：空气、低渗溶液（无菌水）、等渗盐溶液（0. 9%氯化钠）和高渗盐溶液（3. 0%氯化钠）。从10个新鲜小牛股骨中的每一个获得4个骨软骨栓（4.5 mm收获系统），并随机分配至4种储存介质中的1种（N = 40）。使用微型计算机断层扫描来评估每个栓塞的精确直径。时间0扫描后，将每个塞子置于指定的存储介质中，并在约1小时内的3个时间点重新扫描。在潮标近端约1至6 mm处识别出感兴趣区域。定制软件自动计算每个栓塞的直径。所有标本的0时栓塞直径（平均值± 95%置信区间）为4.66 ± 0.01 mm。基线扫描时，任何组之间均无显著差异。未浸没样本的时间0和后续扫描之间也没有显著差异。然而，所有的液体溶液（高渗，等渗，低渗）导致在其基线扫描的直径显着增加（P <0.05），表明原因可能是增加细胞外基质流体pressure. CONCLUSIONSSPlacing一个osteochondrialplug在液体溶液中增加了直径的软骨下骨。在置于溶液中后14分钟内，来自储存介质的尺寸增加似乎趋于平稳。临床相关性：塞直径的增加可能会改变移植物插入的容易程度，可能会增加塞植入期间软骨上的接触压力。
PURPOSEThe purpose of this study was to investigate the effect of differing storage medium on osteochondral plug diameter.METHODSFour storage conditions were evaluated: air, hypotonic solution (sterile water), isotonic saline solution (0.9% sodium chloride), and hypertonic saline solution (3.0% sodium chloride). Four osteochondral plugs were acquired (4.5-mm harvesting system) from each of 10 fresh calf femurs and randomized to 1 of 4 storage media (N = 40). Micro–computed tomography was used to evaluate the precise diameter of each plug. After a time 0 scan, each plug was placed in a designated storage medium and rescanned at 3 time points over approximately 1 hour. A region of interest was identified from approximately 1 to 6 mm proximal to the tidemark. Custom software automatically calculated the diameter of each plug.RESULTSThe time 0 plug diameter (mean ± 95% confidence interval) for all specimens was 4.66 ± 0.01 mm. There were no significant differences between any of the groups at the baseline scan. There were also no significant differences between the time 0 and subsequent scans of the unsubmerged specimens. However, all of the liquid solutions (hypertonic, isotonic, and hypotonic) resulted in a significant increase in diameter from their baseline scans (P < .05), indicating that a cause may be increased extracellular matrix fluid pressure.CONCLUSIONSPlacing an osteochondral plug in a liquid solution increased the diameter of the subchondral bone. Size increase from the storage medium appeared to level off within 14 minutes after placement in solution. Clinical Relevance: Increases in diameter of the plug may alter the ease of insertion of the graft, possibly increasing contact pressure on cartilage during plug implantation.