Satellite cell pool enhancement in rat plantaris muscle by endurance training depends on intensity rather than duration
Satellite cell pool enhancement in rat plantaris muscle by endurance training depends on intensity rather than duration
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DOI：
10.1111/j.1748-1716.2011.02381.x
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发表时间：
2012-05-01
影响因子：
6.3
通讯作者：
Katamoto, S.
中科院分区：
文献类型：
--
作者：
Kurosaka, M.;Naito, H.;Katamoto, S.
目的：卫星细胞数量的增加是维持正常肌肉功能所必需的。耐力训练增强了卫星细胞池。然而，对于增强卫星细胞池，运动强度和运动时间哪个更重要，目前尚不清楚。本研究探讨了不同强度和持续时间耐力训练对大鼠骨骼肌卫星细胞池的影响。方法：选取17周龄雌性Sprague-Dawley大鼠41只，分为对照组（n = 8）、高强度高持续时间组（n = 7）、高强度低持续时间组（n = 8）、低强度高持续时间组（n = 9）和低强度低持续时间组（n = 9）。训练组每周在电动跑步机上锻炼5天，持续10周。训练结束后，麻醉动物，取足底肌肉，称重，分析免疫组织化学和组织化学性质。结果：各组肌肉质量、平均肌纤维面积和每条肌纤维肌核数无显著差异，但高强度组卫星细胞百分比显著高于其他各组（P < 0.05）。结论：耐力训练后骨骼肌卫星细胞池的增加取决于运动强度而不是持续时间。
Aim: Increases in the number of satellite cells are necessary for the maintenance of normal muscle function. Endurance training enhances the satellite cell pool. However, it remains unclear whether exercise intensity or exercise duration is more important to enhance the satellite cell pool. This study examined the effects of different intensity and duration of endurance training on the satellite cell pool in rat skeletal muscle.Methods: Forty-one 17-week-old female Sprague-Dawley rats were assigned to control (n = 8), high intensity and high duration (n = 7), high intensity and low duration (n = 8), low intensity and high duration (n = 9) and low intensity and low duration (n = 9) groups. Training groups exercised 5 days per week on a motor driven treadmill for 10 weeks. After the training period, animals were anaesthetized and the plantaris muscles were removed, weighed and analysed for immunohistochemical and histochemical properties.Results: Although no significant differences were found in muscle mass, mean fibre area and myonuclei per muscle fibre between all groups, the percentage of satellite cells was significantly higher in the high-intensity groups than in the other groups (P < 0.05).Conclusion: Increases in the satellite cell pool of skeletal muscle following endurance training depend on the intensity rather than duration of exercise.