CHOLERA TOXIN-LIKE TOXIN RELEASED BY SALMONELLA SPECIES IN THE PRESENCE OF MITOMYCIN-C
CHOLERA TOXIN-LIKE TOXIN RELEASED BY SALMONELLA SPECIES IN THE PRESENCE OF MITOMYCIN-C
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DOI：
10.1128/iai.30.1.224-230.1980
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发表时间：
1980-01-01
影响因子：
3.1
通讯作者：
PETERSON, JW
中科院分区：
文献类型：
--
作者：
MOLINA, NC;PETERSON, JW
在用丝裂霉素 C (MTC) 进行体外培养期间，几种沙门氏菌血清型释放出数量增加的霍乱毒素样毒素。过滤灭菌的含有毒素的培养物上清液引起中国仓鼠卵巢细胞的伸长，可以通过将上清液加热至100℃来阻止这种伸长。 C 15 分钟或添加混合神经节苷脂或单特异性霍乱抗毒素。当沙门氏菌培养物中未添加 MTC 时，在粗制、未浓缩的培养物上清液中检测到很少或没有检测到毒素。在酪蛋白氨基酸-酵母提取物培养基、Syncase或蛋白胨盐水的摇瓶培养物中，在37℃下，在存在0.5μg MTC/ml的情况下观察到毒素的最佳产生。 C. 肉浸液（心脏浸液和脑心浸液）加 MTC 导致毒素产量较差。培养滤液通常可以按 1:8 稀释，但仍会导致中国仓鼠卵巢细胞的伸长。
Several serotypes of Salmonella released increased amounts of a cholera toxin-like toxin during culture in vitro with mitomycin C (MTC). Filter-sterilized culture supernatants containing the toxin caused elongation of Chinese hamster ovary cells, which could be blocked by heating the supernatants at 100.degree. C for 15 min or by adding mixed gangliosides or monospecific cholera antitoxin. When MTC was not added to the Salmonella cultures, little or no toxin was detected in crude, unconcentrated culture supernatants. Optimal production of toxin was observed in the presence of 0.5 .mu.g of MTC/ml in shake flask cultures of Casamino Acids-yeast extract medium, Syncase or peptone saline at 37.degree. C. Meat infusion media (heart infusion and brain heart infusion) plus MTC resulted in poor toxin yield. Culture filtrates frequently could be diluted 1:8 and still result in elongation of Chinese hamster ovary cells.