CHARACTERIZATION OF THE PREPHENATE DEHYDROGENASE-ENCODING GENE, TYR1, FROM SACCHAROMYCES-CEREVISIAE
CHARACTERIZATION OF THE PREPHENATE DEHYDROGENASE-ENCODING GENE, TYR1, FROM SACCHAROMYCES-CEREVISIAE
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DOI：
10.1016/0378-1119(89)90422-8
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发表时间：
1989-12-28
期刊：
影响因子：
3.5
通讯作者：
FELDMANN, H
中科院分区：
文献类型：
--
作者：
MANNHAUPT, G;STUCKA, R;FELDMANN, H
从酿酒酵母中克隆出酪氨酸生物合成酶预苯脱氢酶（prephenate dehydrogenase, prephenate dehydrogenase）的TYR1基因。含有该基因的DNA片段是一个45 kb的cosmid克隆的一部分，该克隆代表了II染色体上覆盖遗传定位的tyr1位点的区域。已经确定了携带TYR1基因的3.1 kb区域及其邻近区域的核苷酸序列。开放阅读框包含441个密码子，对应编码蛋白约52.2 kDa。典型的nad结合域位于蛋白质的前45个氨基酸中。通过引物延伸，我们发现存在一个转录起点。推测TYR1的表达不受GCN4的一般控制。相反，我们发现这取决于苯丙氨酸的存在与否。这些数据是通过分析含有TYR1启动子片段和CAT报告基因的构建体中的CAT活性获得的。
TYR1, the gene from Saccharomyces cerevisiae, which encodes prephenate dehydrogenase, one of the tyrosine biosynthetic enzymes, has been cloned by complementing a yeast tyr1 mutant strain. The DNA fragment containing the gene is part of a 45-kb cosmid clone which represents a region of chromosome II covering the gentically mapped tyr1 locus. The nucleotide sequence of a 3.1-kb region carrying the TYR1 gene and adjacent regions has been determined. The open reading frame contains 441 codons, corresponding to about 52.2 kDa for the encoded protein. The canonical NAD-binding domain is located within the first 45 amino acids of the protein. By primer extension, we show that there is one transcription start point. Presumably, the expression of TYR1 is not under the general GCN4 control. Instead, we find a dependence on the presence or absence of phenylalanine. These data were obtained by analysing CAT activity in constructs containing promoter fragments of TYR1 and the cat reporter gene.