Continuous Optical Monitoring of Red Blood Cells During a Photosensitization Reaction
Continuous Optical Monitoring of Red Blood Cells During a Photosensitization Reaction
复制标题
DOI：
10.1089/photob.2018.4513
复制
发表时间：
2019-02-01
影响因子：
1.8
通讯作者：
Arai, Tsunenori
中科院分区：
文献类型：
--
作者：
Hamada, Risa;Ogawa, Emiyu;Arai, Tsunenori
背景资料：通过添加红细胞（RBC）作为光敏反应（PR）的研究工具来创建富氧光敏剂溶液。虽然可以使用血红蛋白的光学特性来监测氧水平和反应进程，但以前只能使用间歇测量来完成。高铁血红蛋白浓度随照射时间的增加reported.Objective：我们构建了一个连续的光学测量系统，研究在含有红细胞的光敏剂溶液中的PR的动力学。材料和方法：采用664 nm波长的连续激光，功率密度为60 mW/cm（2），以475-650 nm的宽波段激光作为光路，在1.10mm的比色皿中垂直交叉排列光源，用紫外可见分光光度计测量吸收光谱。该比色皿中的样本由含有30 lg/mL他拉泊芬钠（一种氯光敏剂）的低红细胞压积兔RBC悬浮培养基制备。结果：在PR过程中，氧饱和度持续下降，溶血程度与高铁血红蛋白浓度之间存在一定的关系。结论：我们确定了在PR期间血红蛋白的氧化和氧解吸以及RBC溶血的动力学。我们的测量系统使用RBC中所含血红蛋白的性质，可能有助于持续监测公关动态。
Background: An oxygen-enriched photosensitizer solution was created by the addition of red blood cells (RBCs) as an investigative tool for photosensitization reactions (PRs). Although the oxygen levels and reaction progress can be monitored using the optical characteristics of hemoglobin, previously this has only been done using intermittent measurements. An increase in methemoglobin concentration with irradiation time was reported.Objective: We constructed a continuous optical measurement system to study the dynamics of the PR in a photosensitizer solution containing RBCs. We also measured the relationship between hemolysis and methemoglobin production in the solution.Materials and methods: A 664 nm wavelength continuous laser beam at 60 mW/cm(2) was used to drive the PR, and a broadband (475-650 nm) light beam was used to monitor the absorption spectra during the PR. The light sources were arranged perpendicularly to cross at a 1.10mm cuvette. The sample in this cuvette was prepared from a low-hematocrit rabbit RBC suspension medium containing 30 lg/mL talaporfin sodium, a chlorine photosensitizer. The concentrations of oxygenated hemoglobin, deoxygenated hemoglobin, and methemoglobin were obtained using a multiple regression analysis of the measured spectra.Results: The oxygen saturation decreased continuously during the PR. The relationship between the degree of hemolysis and produced methemoglobin concentration was confirmed.Conclusions: We determined the dynamics of the oxidation and oxygen desorption of hemoglobin, as well as RBC hemolysis, during the PR. Our measurement system, which uses the properties of hemoglobin contained in RBCs, might be useful for continuous monitoring of PR dynamics.