Three-dimensional observation of the mouse embryo by micro-computed tomography: composition of the trigeminal ganglion
Three-dimensional observation of the mouse embryo by micro-computed tomography: composition of the trigeminal ganglion
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DOI：
10.1007/s10266-009-0112-9
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发表时间：
2010-02-01
期刊：
影响因子：
2.5
通讯作者：
Iwasaki, Shin-ichi
中科院分区：
文献类型：
--
作者：
Aoyagi, Hidekazu;Tsuchikawa, Kohzo;Iwasaki, Shin-ichi
本研究的目的是展示一种用于观察小鼠胚胎的微型计算机断层扫描（CT）方法。在交媾后13.0天，小鼠胚胎在4%多聚甲醛中固定24 h，并用四氧化锇整块染色过夜。然后用标准方法将胚胎包埋于石蜡中24h。显微ct分析标本并进行图像处理。含有神经系统和血液系统的器官可以被看作是不同锇染色密度的结果。使用三维技术对三叉神经节成像。用四氧化锇染色显微ct观察胚胎。
The purpose of this study was to demonstrate a micro-computed tomography (CT) method for observations of the mouse embryo. At 13.0 days post-coitum, mouse embryos were fixed in 4% paraformaldehyde for 24 h and stained en bloc by osmium tetroxide overnight. The embryos were then embedded in paraffin using standard methods for 24 h. Specimens were analyzed by micro-CT and image processing was performed. Organs containing nervous and blood systems could be viewed as a result of different osmium-staining densities. The trigeminal ganglion was imaged using three-dimensional techniques. Observation of the embryo was possible by micro-CT with osmium tetroxide staining.