Effect of Electric-Field Intensity on Electropermeabilization and Electrosensitmty of Various Tumor-Cell Lines In Vitro
Effect of Electric-Field Intensity on Electropermeabilization and Electrosensitmty of Various Tumor-Cell Lines In Vitro
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电场强度对体外多种肿瘤细胞系电透性和电敏感性的影响
DOI：
10.3109/15368379809022571
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
G. Serša
中科院分区：
文献类型：
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作者：
Maja Ĉemazˆr;T. Jarm;D. Miklavčič;A. M. Lebar;A. Ihan;N. A. Kopitar;G. Serša
电渗透（电穿孔）是一种广泛用于在体外或体内将各种膜不可渗透分子引入细胞的技术。在这项研究中，我们确定了不同的电场强度对体外各种肿瘤细胞系的电渗透性和电敏感性的影响。为此，我们使用了两种测定：碘化丙啶摄取用于测量细胞电穿孔，以及克隆形成或MTT测定用于测定电敏感性。我们的结果表明，几乎所有测试的细胞系的电渗透发生在600 V/cm。相反，这些细胞系之间存在着明显的电敏感性差异。我们的研究结果可能是非常重要的药理学和生物化学研究在体外，并预测和确定在体内肿瘤反应的电渗透结合化疗药物（电化学疗法）和基因治疗。
Electropermeabilization (electroporation) is a technique widely used to introduce various membrane-impermeable molecules into cells in vitro or in vivo. In this study we determined the effect of different electric-field intensities on electropermeabilization and electrosensitivity of a variety of tumor-cell lines in vitro. For this purpose we used two assays: propidium iodide uptake for measurement of cell electropenneabilization, and the clono-genic or MTT assay for determination of electrosensitivity. Our results showed that electropermeabilization of almost all cell lines tested occurred at 600 V/cm. In contrast, a marked difference in electrosensitivity existed among these cell lines. Our results could be of great importance for pharmacological and biochemical studies in vilro, and for prediction and determination of tumor response in vivo to electropermeabilization combined with chemo-therapeutic drugs (electrochemotherapy) and gene therapy.