MULTIPOLAR SPINDLE 1 (MPS1), a novel coiled-coil protein of Arabidopsis thaliana, is required for meiotic spindle organization
MULTIPOLAR SPINDLE 1 (MPS1), a novel coiled-coil protein of Arabidopsis thaliana, is required for meiotic spindle organization
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多极纺锤体 1 (MPS1) 是拟南芥的一种新型卷曲螺旋蛋白，是减数分裂纺锤体组织所必需的
DOI：
10.1111/j.1365-313x.2009.03929.x
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发表时间：
2009-09-01
期刊：
影响因子：
7.2
通讯作者：
Yang, Zhong-Nan
中科院分区：
文献类型：
--
作者：
Jiang, Hua;Wang, Fen-Fei;Yang, Zhong-Nan
纺锤体在减数分裂过程中对染色体分离至关重要，但高等植物减数分裂纺锤体组织的分子机制尚不清楚。在这里，我们报道了一个植物特异性蛋白，MULTIPOLAR SPINDLE 1 （MPS1）的鉴定和表征，该蛋白参与了拟南芥减数细胞纺锤体的组织。纯合子mps1突变体表现为雄性和雌性不育。光镜观察显示，mps1突变体在花药发育过程中产生了多个不均匀的孢子，其中大部分在后期流产。细胞学分析显示mps1减数细胞染色体分离异常。免疫定位显示mps1减数细胞的双极或多极纺锤体不平等，表明纺锤体异常导致染色体分离紊乱。MPS1编码一种377个氨基酸的蛋白，具有假定的卷曲螺旋基序。原位杂交分析显示MPS1在减数细胞中强烈表达。
P>The spindle is essential for chromosome segregation during meiosis, but the molecular mechanism of meiotic spindle organization in higher plants is still not well understood. Here, we report on the identification and characterization of a plant-specific protein, MULTIPOLAR SPINDLE 1 (MPS1), which is involved in spindle organization in meiocytes of Arabidopsis thaliana. The homozygous mps1 mutant exhibits male and female sterility. Light microscopy showed that mps1 mutants produced multiple uneven spores during anther development, most of which aborted in later stages. Cytological analysis showed that chromosome segregation was abnormal in mps1 meiocytes. Immunolocalization showed unequal bipolar or multipolar spindles in mps1 meiocytes, which indicated that aberrant spindles resulted in disordered chromosome segregation. MPS1 encodes a 377-amino-acid protein with putative coiled-coil motifs. In situ hybridization analysis showed that MPS1 is strongly expressed in meiocytes.