Direct detection of nicotine and cotinine in dusted latent fingermarks of smokers by using hydrophobic silica particles and MS
Direct detection of nicotine and cotinine in dusted latent fingermarks of smokers by using hydrophobic silica particles and MS
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DOI：
10.1002/sia.3112
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发表时间：
2010-05-01
影响因子：
1.7
通讯作者：
Jan, Ma
中科院分区：
文献类型：
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作者：
Benton, Matthew;Rowell, Frederick;Jan, Ma
将炭黑掺杂的亚微米级疏水二氧化硅颗粒与玻璃和金属表面的潜在手印相结合，建立了一种在正离子反射模式下使用表面辅助激光解吸/电离飞行时间质谱（SALDI-TOF-MS）检测尼古丁和可替宁的简单方法。表面上的灰尘可以定位标记，以进行常规的细节识别，但颗粒也可以作为激光解吸/电离增强剂（LDI），相当于在基质辅助LDI- tof - ms （MALDI-TOF-MS）中使用的标准基质增强剂2,5-二羟基苯甲酸。该方法已应用于金属和玻璃表面吸烟者潜在手印的分析。金属表面的分析是在对预喷涂的指纹进行直接质谱分析后进行的，而玻璃表面的分析是在使用商用胶带进行提升后进行的，然后对这些预喷涂的指纹进行质谱分析。在所有吸烟者中，在指纹中发现了一个主要的峰在m/z 163（尼古丁+1）和一个较弱的峰在m/z 161（可能是阿那他宾+1），在某些情况下观察到额外的峰在177（可替宁+1）和199（可能是可替宁+Na）。当观察到每种化合物的特征碎片模式时，使用SALDI-TOF-MS-MS在高能碰撞诱导解离后证实了吸烟者标记中尼古丁和可替宁的存在。版权所有John Wiley & Sons， Ltd. 2009
Sub-micron sized hydrophobic silica particles doped with carbon black have been employed with latent fingermarks on glass and metal surfaces to develop a simple method for detecting nicotine and cotinine using surface assisted laser desorption/ionisation-time of flight-mass spectrometry (SALDI-TOF-MS) in positive ion reflectron mode. Dusting of surfaces enables location of marks for conventional identification of details but the particles also act as a laser desorption/ionisation enhancing agent (LDI) equivalent to a standard matrix enhancer 2,5-dihydroxybenzoic acid used in matrix assisted LDI-TOF-MS (MALDI-TOF-MS). The method has been applied in the analysis of smokers' latent fingermarks on metal and glass surfaces. The metal surfaces were analysed following direct MS analysis of the pre-dusted prints, while the glass surfaces were analysed following lifting using commercial tape, and then MS analysis of these pre-dusted prints. In all cases of smokers, a major peak at m/z 163 (nicotine+1) and a less intense peak at m/z 161 (possibly anatabine+1) were found in the prints and in some cases additional peaks at 177 (cotinine+1) and 199 (possibly cotinine+Na) were observed. The presence of nicotine and cotinine in smokers' marks was confirmed using SALDI-TOF-MS-MS following high energy collision-induced dissociation when characteristic fragmentation patterns were observed for each compound. Copyright (C) 2009 John Wiley & Sons, Ltd.