Knockdown of a Nicotinic Acetylcholine Receptor Subunit Gene Bdorβ1 Decreases Susceptibility to Oxa-Bridged trans- instead of cis-Nitromethylene Neonicotinoid Insecticides in Bactrocera dorsalis.
Knockdown of a Nicotinic Acetylcholine Receptor Subunit Gene Bdorβ1 Decreases Susceptibility to Oxa-Bridged trans- instead of cis-Nitromethylene Neonicotinoid Insecticides in Bactrocera dorsalis.
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DOI：
10.1021/acs.jafc.2c04709
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发表时间：
2022-10
影响因子：
6.1
通讯作者：
Guorui Yuan;Mengling Chen;Meng-Lan Peng;Wei Lei;Li-Wei Meng;W. Dou;Jinjun Wang
中科院分区：
文献类型：
--
作者：
Guorui Yuan;Mengling Chen;Meng-Lan Peng;Wei Lei;Li-Wei Meng;W. Dou;Jinjun Wang
烟碱型乙酰胆碱受体(NAChRs)是一种配体门控离子通道，在突触胆碱能传递中介导乙酰胆碱的快速作用。昆虫的nAChRs是几类杀虫剂的靶标。在这里，我们从一种毁灭性的害虫--桔小实蝇中克隆并鉴定了编码nAChRβ1亚基(BDORβ1)的全长基因。BDORβ1的氨基酸序列与其他昆虫β1亚基有很高的同源性。双次注射dsBdorβ1可降低Bdorβ1的表达，进而显著降低对氧桥联的反式取代顺式亚甲基新烟碱的敏感性。我们的结果支持BDORβ1参与了桔小实蝇对氧桥联的反式而不是顺式亚硝基新烟碱类化合物的敏感性，并暗示这两类新烟碱类化合物可能作用于不同的nAChR亚型。
Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels that mediate the fast action of acetylcholine in synaptic cholinergic transmissions. Insect nAChRs are the target of several classes of insecticides. Here, the full-length cDNA encoding a nAChR beta1 subunit (Bdorβ1) was identified and characterized from a destructive pest, Bactrocera dorsalis. The amino acid sequence of Bdorβ1 shows high identities to other insect nAChRs β1 subunits. Double injection of dsBdorβ1 reduced the expression of Bdorβ1 and in turn significantly decreased susceptibility to oxa-bridged trans- instead of cis-nitromethylene neonicotinoids. Our results support the involvement of Bdorβ1 in the susceptibility of B. dorsalis to oxa-bridged trans- instead of cis-nitromethylene neonicotinoids and imply that these two classes of neonicotinoids might be acting at different nAChR subtypes.