Polyphasic circadian neural circuits drive differential activities in multiple downstream rhythmic centers.
Polyphasic circadian neural circuits drive differential activities in multiple downstream rhythmic centers.
复制标题
多相昼夜节律神经回路驱动多个下游节律中心的差异活动。
DOI：
10.1016/j.cub.2022.12.025
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Taghert,PaulH
中科院分区：
文献类型：
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作者：
Liang,Xitong;Holy,TimothyE;Taghert,PaulH
生物钟将各种行为，如运动活动、睡眠/觉醒、进食和交配，与一天中最具适应性的时间联系在一起。起搏器回路中的节律信息是如何转化为神经元输出的，目前还不清楚。在这里，我们在果蝇大脑中使用全脑，24-hin内钙成像，并在已识别的多巴胺能（DA）和肽能神经分泌（NS）神经元簇中寻找昼夜节律活动。这种节律是普遍存在的，并由周期依赖的时钟活动施加在约150个细胞的昼夜节律起搏器网络中。节律表现为早晨(M)、晚上(E)或中午（MD）阶段。不同亚群的昼夜节律起搏器将神经活动节律施加到不同的下游非时钟神经元上。典型的M和E起搏器的输出汇聚来调节DA-PPM3和DA-PAL神经元。E起搏器调节夜间活动的DA-PPL1神经元。除了这些典型的M和E振荡外，我们还提供了在中午发生的第三个专用阶段的证据：l-LNv起搏器呈现MD活性峰值，它们调节MD活性的DA-PPM1/2神经元和三种不同的NS细胞类型。因此，果蝇昼夜节律起搏器网络是一个多相节律发生器。它呈现出专用的M、E和MD相，这些相在功能上被转导为神经元输出，在下游回路中组织各种日常活动模式。
Circadian clocks align various behaviors such as locomotor activity, sleep/wake, feeding, and mating to times of day that are most adaptive. How rhythmic information in pacemaker circuits is translated to neuronal outputs is not well understood. Here, we used brain-wide, 24-hin vivocalcium imaging in theDrosophilabrain and searched for circadian rhythmic activity among identified clusters of dopaminergic (DA) and peptidergic neurosecretory (NS) neurons. Such rhythms were widespread and imposed by the PERIOD-dependent clock activity within the ∼150-cell circadian pacemaker network. The rhythms displayed either a morning (M), evening (E), or mid-day (MD) phase. Different subgroups of circadian pacemakers imposed neural activity rhythms onto different downstream non-clock neurons. Outputs from the canonical M and E pacemakers converged to regulate DA-PPM3 and DA-PAL neurons. E pacemakers regulate the evening-active DA-PPL1 neurons. In addition to these canonical M and E oscillators, we present evidence for a third dedicated phase occurring at mid-day: the l-LNv pacemakers present the MD activity peak, and they regulate the MD-active DA-PPM1/2 neurons and three distinct NS cell types. Thus, theDrosophilacircadian pacemaker network is a polyphasic rhythm generator. It presents dedicated M, E, and MD phases that are functionally transduced as neuronal outputs to organize diverse daily activity patterns in downstream circuits.