Mapping diploid wheat homologues of Arabidopsis seed ABA signaling genes and QTLs for seed dormancy
Mapping diploid wheat homologues of Arabidopsis seed ABA signaling genes and QTLs for seed dormancy
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DOI：
10.1007/s00122-007-0502-8
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发表时间：
2007-03
影响因子：
5.4
通讯作者：
S. Nakamura;T. Komatsuda;H. Miura
中科院分区：
文献类型：
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作者：
S. Nakamura;T. Komatsuda;H. Miura
胚对脱落酸（阿坝）的敏感性是小麦种子休眠的关键因素之一。拟南芥中已分离出许多阿坝信号基因，而小麦中只有少数同源基因被鉴定。本研究利用一粒小麦（Triticum monococcum，Tm）与黑穗小麦（T. boeoticum（Tb）. TmVP 1、TmABF、TmABI 8和TmERA 1位于3号染色体上，TmERA 3位于5Am的着丝粒区。在连续两年的试验中，检测到1个位于5Am长臂上的主效QTL，2个位于3Am长臂上的次效QTL，1个位于4Am长臂上的次效QTL。5AmQTL解释了20-27%的表型变异，其他3个QTL各解释了约10%的表型变异。TmABF 1和TmABI 8位点的图谱位置与位于3Am的两个QTL的LOD峰值相吻合，表明这两个QTL可能是种子休眠QTL的候选基因。此外，我们还发现了两个SNP导致TmABF中Tb和Tm之间的氨基酸替换。比较大麦种子休眠性QTL的标记位置发现，5 Am上最大的QTL可能与大麦种子休眠性QTL SD 1直系同源，而与相应的大麦SD 2位点似乎没有直系同源的QTL。
Abscisic acid (ABA) sensitivity in embryos is one of the key factors in the seed dormancy of wheat. Many ABA signaling genes have been isolated inArabidopsis, while only a few wheat homologues have been identified. In the present study, diploid wheat homologues toArabidopsisABA signaling genes were identified byin silicoanalysis, and mapped them using a population of diploid wheat recombinant inbred lines derived from a cross betweenTriticum monococcum(Tm) andT. boeoticum(Tb). Four diploid wheat homologues,TmVP1,TmABF,TmABI8andTmERA1were located on chromosome 3AmandTmERA3was on the centromere region of chromosome 5Am. In two consecutive year trials, one major QTL on the long arm of 5Am, two minor QTLs on the long arm of 3Amand one minor QTL on the long arm of 4Amwere detected. The 5AmQTL explained 20–27% of the phenotypic variations and the other three QTLs each accounted for approximately 10% of the phenotypic variations. Map positions of the loci ofTmABFandTmABI8matched the LOD peaks of the two QTLs on 3Am, indicating that these two homologues are possible candidate genes for seed dormancy QTLs. Moreover, we have found two SNPs result in amino acid substitutions inTmABFbetween Tb and Tm. Comparison of the marker positions of QTLs for seed dormancy of barley revealed that the largest QTL on 5Ammay be orthologous to the barley seed dormancy QTL, SD1, whereas there seems no orthologous QTL to the corresponding barley SD2 locus.