Accessory Gene Regulator (agr) Allelic Variants in Cognate Staphylococcus aureus Strain Display Similar Phenotypes.
Accessory Gene Regulator (agr) Allelic Variants in Cognate Staphylococcus aureus Strain Display Similar Phenotypes.
复制标题
同源金黄色葡萄球菌菌株中的辅助基因调节子 (agr) 等位变体表现出相似的表型
DOI：
10.3389/fmicb.2022.700894
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发表时间：
2022
影响因子：
5.2
通讯作者：
Li S
中科院分区：
文献类型：
--
作者：
Tan L;Huang Y;Shang W;Yang Y;Peng H;Hu Z;Wang Y;Rao Y;Hu Q;Rao X;Hu X;Li M;Chen K;Li S
辅助基因调节剂（AGR）群体感应系统是金黄色葡萄球菌的重要全球调节系统，并有助于其致病性。基于AGRB，AGRD和AGRC的氨基酸多态性，金黄色葡萄球菌AGR系统分为四个AGR组。 AGR激活是特异性的，导致四个AGR组之间的AGR活性和致病性变化。带有不同AGR系统的菌株总是具有不同的表型。在本报告中，我们分别与其他三个AGR等位基因交换了特定金黄色葡萄球菌的AGR系统，并评估了这些先天性菌株的相应表型。替代AGR系统导致与亲本菌株相比，溶血活性，蛋白质表达和毒力基因表达的显着变化。有趣的是，我们发现在相同菌株背景下这些AGR先生菌株的生物学特征彼此高度相似，并且AGR系统的等位基因依赖性差异被削弱。这些发现表明，金黄色葡萄球菌的等位基因依赖性AGR偏见还取决于AGRB，AGRD和AGRC的多态性以外的某些因素。未来的研究可能揭示了提高我们对AGR网络的理解的新型机制。
The accessory gene regulator (agr) quorum-sensing system is an important global regulatory system of Staphylococcus aureus and contributes to its pathogenicity. The S. aureus agr system is divided into four agr groups based on the amino acid polymorphisms of AgrB, AgrD, and AgrC. The agr activation is group-specific, resulting in variations in agr activity and pathogenicity among the four agr groups. Strains with divergent agr system always have different phenotypes. In the present report, we, respectively, exchanged the agr system of a certain S. aureus with other three agr alleles and assessed the corresponding phenotypes of these congenic strains. Replacement of the agr system led to significant variations in hemolytic activity, protein expression, and virulence gene expression comparing with that of the parental strain. Interestingly, we found that the biological characteristics of these agr congenic strains in the same strain background were highly similar to each other, and the allele-dependent differences of the agr systems were weakened. These findings indicate that the allele-dependent agr predilections of S. aureus are determined by some factors in addition to the polymorphisms of AgrB, AgrD, and AgrC. Future studies may reveal the novel mechanism to improve our understanding of the agr network.