Melatonin inhibits high voltage activated calcium currents in cultured rat dorsal root ganglion neurones
Melatonin inhibits high voltage activated calcium currents in cultured rat dorsal root ganglion neurones
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DOI：
10.1016/s0304-3940(01)02188-7
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发表时间：
2001-11-02
影响因子：
2.5
通讯作者：
Kelestimur, H
中科院分区：
文献类型：
--
作者：
Ayar, A;Martin, DJ;Kelestimur, H
采用全细胞膜片钳和fura-2荧光比Ca2+成像技术研究了褪黑素对培养的新生大鼠背根神经节（DRG）神经元中高压活化钙通道（HVACC）和细胞内游离Ca2+浓度的影响。对HVACC进行药理学和生物物理分离，并研究褪黑素的作用。细胞外应用褪黑素以剂量依赖的方式抑制HVACC。在钙成像实验中，应用含有30 mM KCI的细胞外记录介质引起细胞内游离Ca2+的增加，这依赖于外部Ca2+离子。低剂量（10毫微克）和高剂量（100毫微克）褪黑素预处理均可防止这种增加。本研究结果表明，松果体褪黑激素对培养大鼠DRG神经元电压依赖性钙进入有抑制作用。(C) 2001爱思唯尔科学爱尔兰有限公司版权所有。
The actions of melatonin on high-voltage activated calcium channels (HVACC) and intracellular free Ca2+ concentration in cultured dorsal root ganglion (DRG) neurones from neonatal rats were investigated using the whole-cell patch clamp and the fura-2 fluorescence ratio Ca2+-imaging techniques. HVACC were pharmacologically and biophysically isolated and the effects of melatonin were investigated. Extracellular application of melatonin inhibited HVACC in a dose dependent manner. In calcium imaging experiments, application of extracellular recording medium containing 30 mM KCI evoked increases in intracellular free Ca2+ that were dependent upon external Ca2+ ions. This increase was prevented by both low (10 muM) and high dose (100 muM) of melatonin pre-treatment. The results of this study indicate that the pineal hormone melatonin has inhibitory actions on voltage dependent calcium entry in cultured rat DRG neurones. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.