3D printing of PCL-ceramic composite scaffolds for bone tissue engineering applications
3D printing of PCL-ceramic composite scaffolds for bone tissue engineering applications
复制标题
用于骨组织工程应用的 PCL-陶瓷复合支架的 3D 打印
DOI：
--
复制
发表时间：
2023
影响因子：
8.4
通讯作者：
S. Desai
中科院分区：
文献类型：
--
作者：
Santosh Kumar Parupelli;Sheikh Saudi;N. Bhattarai;S. Desai
采用三维打印技术制备了聚己内酯（PCL）/磷酸钙镁（CMP）生物陶瓷复合骨组织工程支架材料。使用定制3D打印机制造四组支架，即PMC-0、PMC-5、PMC-10和PMC-15。对支架的流变学、表面形貌和润湿性进行了表征。PMC-0支架显示出更光滑的表面纹理，并且复合支架的陶瓷含量的增加表现出更粗糙的结构。与对照PMC-0相比，复合支架的亲水性显著增强。研究陶瓷含量对成纤维细胞NIH/3 T3在复合支架中生物活性的影响。分别通过比较乳酸脱氢酶（LDH）和Alcohol Blue（AB）比色测定的结果来评价细胞活力和毒性研究。活-死细胞试验表明，与对照样品相比，试验样品在第3天具有超过100%的活细胞的生物相容性。LDH释放表明复合支架促进了细胞的附着和增殖。在这项研究中，定制的复合3D支架的制造不仅模仿了天然骨组织基质的粗糙纹理结构，孔隙率和化学成分，而且还作为有利于骨细胞生长的可溶性钙和镁离子的来源。因此，这些3D打印支架提供了一个理想的微环境，以促进生物矿化，并可能是一种新的有效方法，用于制备适合骨组织工程的结构。
Three-dimensional (3D) printing was utilized for the fabrication of a composite scaffold of poly(ε-caprolactone) (PCL) and calcium magnesium phosphate (CMP) bioceramics for bone tissue engineering application. Four groups of scaffolds, that is, PMC-0, PMC-5, PMC-10, and PMC-15, were fabricated using a custom 3D printer. Rheology analysis, surface morphology, and wettability of the scaffolds were characterized. The PMC-0 scaffolds displayed a smoother surface texture and an increase in the ceramic content of the composite scaffolds exhibited a rougher structure. The hydrophilicity of the composite scaffold was significantly enhanced compared to the control PMC-0. The effect of ceramic content on the bioactivity of fibroblast NIH/3T3 cells in the composite scaffold was investigated. Cell viability and toxicity studies were evaluated by comparing results from lactate dehydrogenase (LDH) and Alamar Blue (AB) colorimetric assays, respectively. The live-dead cell assay illustrated the biocompatibility of the tested samples with more than 100% of live cells on day 3 compared to the control one. The LDH release indicated that the composite scaffolds improved cell attachment and proliferation. In this research, the fabrication of a customized composite 3D scaffold not only mimics the rough textured architecture, porosity, and chemical composition of natural bone tissue matrices but also serves as a source for soluble ions of calcium and magnesium that are favorable for bone cells to grow. These 3D-printed scaffolds thus provide a desirable microenvironment to facilitate biomineralization and could be a new effective approach for preparing constructs suitable for bone tissue engineering.