Expression of MxA protein in blood lymphocytes discriminates between viral and bacterial infections in febrile children
Expression of MxA protein in blood lymphocytes discriminates between viral and bacterial infections in febrile children
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DOI：
10.1203/00006450-199705000-00008
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发表时间：
1997-05-01
影响因子：
3.6
通讯作者：
Makela, MJ
中科院分区：
文献类型：
--
作者：
Halminen, M;Ilonen, J;Makela, MJ
干扰素（IFN）是由病毒诱导产生的，是机体防御病毒感染的重要组成部分。IFN的抗病毒作用是由几种IFN诱导的基因产物介导的，其中之一是Mx蛋白。为了评估淋巴细胞中MxA蛋白表达是否可以作为急性发热性疾病儿童内源性IFN产生的指标，我们通过流式细胞术分析了疾病急性期外周血淋巴细胞中MxA蛋白水平。实验室确认的病毒感染儿童[呼吸道合胞病毒（RSV）21例，腺病毒10例，轮状病毒5例，流感，单纯疱疹病毒或EBV 7例]的（p < 0.002）MxA蛋白水平（不同病毒组的中值荧光范围为707至765）与细菌感染儿童（n = 12，中值荧光548）相比。为了进一步表征感染期间MxA蛋白的表达，用外源性IFN-α体外刺激来自41名患者的细胞，并测定MxA蛋白的表达水平。与病毒感染组相比，细菌感染组中MxA染色水平的升高显著更高（p < 0.005），进一步表明病毒感染患者体内MxA蛋白水平已经升高。这项研究表明，MxA蛋白表达水平升高可用于细菌与病毒性疾病的发热儿童的鉴别诊断。
Interferons (IFNs), which are induced by viruses, form an essential part of host's defense systems against viral infections. The antiviral actions of IFNs are mediated by several IFN-inducible gene products, one of which is Mx protein. To evaluate whether MxA protein expression in lymphocytes could function as an indicator of endogenous IFN production in children with acute febrile illness, we analyzed MxA protein levels in peripheral blood lymphocytes by flow cytometry in the acute phase of the disease. Children with a laboratory-confirmed viral infection [respiratory syncytial virus (RSV) in 21, adenovirus in 10, rotavirus in 5, and influenza, herpes simplex, or EBV in 7 other cases] had significantly higher (p < 0.002) MxA protein levels (median fluorescences in different virus groups ranged from 707 to 765) compared with children with a bacterial infection (n = 12, median fluorescence 548). To characterize further MxA protein expression during infections, cells from 41 patients were stimulated in vitro with exogenous IFN-alpha, and the level of MxA protein expression was determined. The rise in MxA staining levels was significantly higher in the group with bacterial infections compared with those with viral infection (p < 0.005), further indicating that the MxA protein levels were already elevated in vivo in patients with viral infections. This study suggests that elevated MxA protein expression levels can be used in the differential diagnosis of bacterial versus viral disease in febrile children.