Organic anion-transporting polypeptides are involved in the elimination of insecticides from the red flour beetle, Tribolium castaneum
Organic anion-transporting polypeptides are involved in the elimination of insecticides from the red flour beetle, Tribolium castaneum
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DOI：
10.1007/s10340-020-01317-4
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发表时间：
2021-01-21
影响因子：
4.8
通讯作者：
Merzendorfer, Hans
中科院分区：
文献类型：
--
作者：
Roesner, Janin;Tietmeyer, Johanne;Merzendorfer, Hans
有机阴离子转运多肽（oats）是一种完整的膜转运蛋白，可介导人体对多种底物的细胞摄取。ooatp在昆虫中的功能研究较少，了解也很少。大量对昆虫有潜在毒性的化合物是有机阴离子，包括次生植物和杀虫剂衍生的代谢物。一些昆虫OATP基因在代谢、神经保护和排泄组织中表达，它们与已知参与解毒和排泄的基因一起共表达。因此，已经提出了oops在消除杀虫剂方面的作用，但尚未得到实验证明。本研究旨在鉴定影响红粉甲虫（Tribolium castaneum）对杀虫剂耐受性的oops。红粉甲虫是一种基因组模式种和储粮害虫。我们测定了TcOATP基因在不同组织和发育阶段的表达谱，并分析了RNAi表型。我们发现一些TcOATP基因在相关组织中有特别高的转录水平，TcOATP4-C1的敲低会导致幼虫-蛹蜕皮过程中严重的发育缺陷。然后，我们将幼虫暴露于不同化学无关的杀虫剂中，并分析转录水平和死亡率。一些基因在杀虫剂处理下特异性上调，当特定的tcoatp被沉默后，使用某些杀虫剂后观察到的死亡率显着增加。通过对castaneum应用系统性RNAi，我们首次提供了oops参与杀虫剂消除的证据，因此可能有助于杀虫剂抗性，这已成为农业和林业中日益严重的问题。
Organic anion-transporting polypeptides (OATPs) are integral membrane transporters that mediate cellular uptake of a broad range of substrates in humans. The functions of OATPs in insects are less well investigated and only poorly understood. A large number of compounds potentially toxic to insects are organic anions that include secondary plant and insecticide-derived metabolites. Some insect OATP genes are expressed in metabolic, neuroprotective and excreting tissues, and they are co-expressed together with genes known to be involved in detoxification and excretion. Therefore, a role in the elimination of insecticides has been proposed for OATPs, but experimental proof was pending. The aim of this study was to identify OATPs that affect tolerance to insecticides in the red flour beetle, Tribolium castaneum, a genomic model species and stored product pest. We determined expression profiles of TcOATP genes in different tissues and developmental stages and analyzed RNAi phenotypes. We found that some TcOATP genes had particularly high transcript levels in relevant tissues and that knockdown of TcOATP4-C1 led to sever developmental defects during larval-pupal molt. Then, we exposed the larvae to different chemically unrelated insecticides and analyzed transcript levels and mortalities. Some genes were specifically upregulated in response to insecticide treatment, and mortalities observed after administering certain insecticides were significantly increased when specific TcOATPs were silenced. By applying systemic RNAi in T. castaneum, we provide first evidence that OATPs are involved in the elimination of insecticides and hence may contribute to insecticide resistance, which becomes an increasingly serious problem in agriculture and forestry.[GRAPHICS].