Requirements of fission yeast septins for complex formation, localization, and function
Requirements of fission yeast septins for complex formation, localization, and function
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DOI：
10.1091/mbc.e04-07-0640
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发表时间：
2004-12-01
影响因子：
3.3
通讯作者：
Gould, KL
中科院分区：
文献类型：
--
作者：
An, HB;Morrell, JL;Gould, KL
在许多真核生物中，Septins是对胞质分裂重要的GTP结合蛋白。粟酒裂殖酵母基因组序列预测了参与胞质分裂的五种酿酒酵母隔膜中的四种的直向同源物，这些被命名为Spns 1 -4p。spns 1 -4不是必需基因，这允许应用遗传学和蛋白质组学相结合的方法来确定它们的功能关系。我们的研究结果表明，Spns 1 -4p在整个间期以类似于8.5S复合物的弥散定位存在，其中每个septin的两个拷贝以Spn 3 p-Spn 4p-Spn 1 p-Spn 2 p的顺序连接在一起。隔蛋白募集到细胞的中间区域在遗传上与环形成是可分离的，并且虽然它通常限于有丝分裂，但它可以在没有激活有丝分裂细胞周期机制的情况下被促进。聚结成环状结构需要Spn 1 p和Spn 4p与至少一个其他隔蛋白亚基以及通常限于有丝分裂的Mid 2 p的表达相关联。本研究确立了septin复合物在体内组织的功能要求。
Septins are GTP binding proteins important for cytokinesis in many eukaryotes. The Schizosaccaromyces pombe genome sequence predicts orthologues of four of five Saccharomyces cerevisiae septins involved in cytokinesis and these are named Spns1-4p. That spns1-4 are not essential genes permitted the application of a combined genetic and proteomics approach to determine their functional relationships. Our findings indicate that Spns1-4p are present throughout interphase as a diffusely localized similar to8.5S complex containing two copies of each septin linked together as a chain in the order Spn3p-Spn4p-Spn1p-Spn2p. Septin recruitment to the medial region of the cell is genetically separable from ring formation, and whereas it is normally restricted to mitosis, it can be promoted without activation of the mitotic cell cycle machinery. Coalescence into ring structures requires Spn1p and Spn4p associate with at least one other septin subunit and the expression of Mid2p that is normally restricted to mitosis. This study establishes the functional requirements for septin complex organization in vivo.