CO2-driven ocean acidification repressed the growth of adult sea urchin Strongylocentrotus intermedius by impairing intestine function
CO2-driven ocean acidification repressed the growth of adult sea urchin Strongylocentrotus intermedius by impairing intestine function
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二氧化碳驱动的海洋酸化通过损害肠道功能抑制成年海胆 Strongylocentrotus intermedius 的生长
DOI：
10.1016/j.marpolbul.2020.110944
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发表时间：
2020
影响因子：
5.8
通讯作者：
Chang Yaqing
中科院分区：
文献类型：
--
作者：
Zhan Yaoyao;Cui Dongyao;Xing Dongfei;Zhang Jun;Zhang Weijie;Li Yingying;Li Cong;Chang Yaqing
利用在中国黄海北部养殖的中间强海胆来评估慢性二氧化碳驱动的海洋酸化（OA）对成年海胆的影响。根据政府间气候变化专门委员会 (IPCC) 的预测，采用了当前的自然海水条件 (pHNBS=8.10±0.03) 和三个实验室控制的 OA 条件 (OA1, ΔpHNBS=−0.3单位；OA2, ΔpHNBS=−0.4单位；OA3, ΔpHNBS=−0.5单位)。经过 60 天的培养，我们的结果表明（1）OA 显着抑制成虫的生长。中间体； (2)食物消耗量随pH值下降呈减少趋势； (3)肠道形态发生改变，酸化条件下肠道纤维素酶和脂肪酶活性降低； (4)两个免疫相关基因SiTNF14和SiTGF-β表达水平发生改变； (5) 与对照组相比，所有 OA 处理中糖酵解途径和三羧酸循环 (TAC) 的限速酶活性均发生变化。
Strongylocentrotusintermediuscultured in the northern Yellow Sea in China was utilized to evaluate the effects of chronic CO2-driven ocean acidification (OA) on adult sea urchins. Based on the projection of the Intergovernmental Panel on Climate Change (IPCC), present natural seawater conditions (pHNBS= 8.10 ± 0.03) and three laboratory-controlled OA conditions (OA1, ΔpHNBS= − 0.3 units; OA2, ΔpHNBS= − 0.4 units; OA3, ΔpHNBS= − 0.5 units) were employed. After 60-day incubation, our results showed that (1) OA significantly repressed the growth of adultS. intermedius; (2) food consumption tended to be decreased with pH decline; (3) intestinal morphology was changed, and activities of intestinal cellulase and lipase were decreased under acidified conditions; (4) expression levels of two immune-related genes (SiTNF14andSiTGF-β) were altered; (5) rate-limiting enzyme activities of the glycolytic pathway and tricarboxylic acid cycle (TAC) were changed in all OA treatments compared to those of controls.