Microfilament assembly and cortical granule distribution during maturation, parthenogenetic activation and fertilisation in the porcine oocyte
Microfilament assembly and cortical granule distribution during maturation, parthenogenetic activation and fertilisation in the porcine oocyte
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DOI：
10.1017/s0967199400003026
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发表时间：
1996-05-01
期刊：
影响因子：
1.7
通讯作者：
Chung, KS
中科院分区：
文献类型：
--
作者：
Kim, NH;Day, BN;Chung, KS
在这项研究中，我们成像了猪卵母细胞成熟、孤雌激活和体外受精过程中微丝组装和皮质颗粒分布的整体变化，并检测了微丝抑制剂对皮质颗粒分布的影响。用荧光标记的凝集素和罗丹明标记的鬼臼毒素在激光扫描共聚焦显微镜下对微丝组装和皮质颗粒分布进行成像。在生发泡阶段，皮质颗粒细胞器位于细胞皮质周围，在卵膜上呈较宽的区域。在细胞皮质周围广泛而均匀的区域也可观察到微丝。生发泡破裂后，微丝聚集在浓缩的染色质中，皮质中可见皮质颗粒。用细胞松弛素B治疗可抑制微丝聚合，并阻止皮质颗粒向皮质移动。精子穿透后的皮质颗粒渗出均匀分布于整个卵黄周间隙。这些结果表明，微丝组装参与了成熟和受精过程中皮质颗粒的分布、运动和胞吐。
In this study we imaged integral changes in microfilament assembly and cortical granule distribution, and examined effects of microfilament inhibitor on the cortical granule distribution during oocyte maturation, parthenogenetic activation and in vitro fertilisation in the pig. The microfilament assembly and cortical granule distribution were imaged with fluorescent-labelled lectin and rhodamine-labelled phalloidin under laser scanning confocal microscopy. At the germinal vesicle stage, cortical granule organelles were located around the cell cortex and were present as a relatively wide area on the oolemma. Microfilaments were also observed in a wide uniform area around the cell cortex. Following germinal vesicle breakdown, microfilaments concentrated in the condensed chromatin and cortical granules were observed in the cortex. Treatment with cytochalasin B inhibited microfilament polymerisation and prevented movement of cortical granules to the cortex. Cortical granule exudation following sperm penetration was evenly distributed in the entire perivitelline space. These results suggest that the microfilament assembly is involved in the distribution, movement and exocytosis of cortical granules during maturation and fertilisation.