Expression and the biological activities of insulin-like growth factor-binding protein related protein 1 in rat uterus during the periimplantation period
Expression and the biological activities of insulin-like growth factor-binding protein related protein 1 in rat uterus during the periimplantation period
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DOI：
10.1210/en.2004-0415
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发表时间：
2004-11-01
期刊：
影响因子：
4.8
通讯作者：
Kogo, H
中科院分区：
文献类型：
--
作者：
Tamura, K;Hara, T;Kogo, H
胰岛素样生长因子结合蛋白相关蛋白1（IGFBP-rP 1）在着床前后大鼠子宫中高度表达。在本研究中，我们确定了IGFBP-rP 1 mRNA在着床周围的定位，并评估了重组IGFBP-rP 1对妊娠早期培养的子宫内膜细胞增殖和前列环素（PGI（2））产生能力的影响。IGFBP-rP 1 mRNA在靠近着床点平滑肌的子宫内膜间质细胞中高水平表达，但在胚胎周围的蜕膜区不表达。IGFBP-rP 1在延迟着床妊娠模型中也有不同程度的表达，但随着蜕膜化模型中蜕膜组织的形成，IGFBP-rP 1 mRNA的表达水平降低。重组IGFBP-rP 1可抑制体外培养的子宫内膜间质细胞的增殖，并使其阻滞于细胞周期的G（1）期。此外，IGFBP-rP 1显著刺激子宫肌层细胞中PGI（2）的合成和环氧合酶II mRNA的表达，这两者都是成功植入的必要分子。这些数据表明，IGFBP-rP 1是一种着床相关蛋白，在围着床期调节大鼠子宫细胞的增殖及其PGI的产生。
IGF binding protein-related protein 1 (IGFBP-rP1) is highly expressed in the rat uterus around the time of implantation. In the present study, we determined the periimplantation localization of IGFBP-rP1 mRNA and assessed the effects of recombinant IGFBP-rP1 on the proliferative and prostacyclin (PGI(2))-producing abilities of cultured endometrial cells early in pregnancy. IGFBP-rP1 mRNA was detected at high levels in endometrial stromal cells close to the smooth muscle of interimplantation sites around the time of implantation but absent from decidual zones surrounding the embryo. Differential uterine IGFBP-rP1 expression was also recognized in the delayed implanting pregnant model, but the level of mRNA decreased as decidual tissues formed in the decidualization model. Recombinant IGFBP-rP1 inhibited the proliferation of endometrial stromal cells in vitro and arrested them in the G(1) phase of the cell cycle. Furthermore, IGFBP-rP1 significantly stimulated PGI(2) synthesis and cyclooxygenase II mRNA expression in myometrial cells, both of which are essential molecules for successful implantation. These data suggest that IGFBP-rP1 is an implantation-associated protein and that it modulates the proliferation of rat uterine cells and their production of PGI(2) during the periimplantation period.