Immunohistochemical expression of inhibin/activin subunits in epithelial and granulosa cell tumours of the ovary
Immunohistochemical expression of inhibin/activin subunits in epithelial and granulosa cell tumours of the ovary
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DOI：
10.1002/(sici)1096-9896(199704)181:4
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发表时间：
1997-04-01
影响因子：
7.3
通讯作者：
Wells, M
中科院分区：
文献类型：
--
作者：
Arora, DS;Cooke, IE;Wells, M
二聚体抑制素-A最近被一种灵敏的两点酶联免疫吸附试验证明是卵巢颗粒细胞瘤的一个有用的肿瘤标志物。在一些卵巢恶性上皮性肿瘤患者中，它也升高。为了确定抑制素在卵巢肿瘤中表达的准确亚基，用免疫组织化学方法对6个颗粒细胞和9个上皮性肿瘤的3个单抗R1、E4和INPRO(分别针对αC亚基、βA亚基和原α亚基)进行了评价。所有颗粒细胞瘤的E4和R1染色均为阳性，提示二聚体抑制素-A的表达；在两名术前检测血清水平的患者中，他们的水平升高。肿瘤对INPRO的反应也很弱，这表明存在阿尔法亚单位的前体形式。8例卵巢恶性上皮性肿瘤仅表达βA亚单位，E4识别，而1例肿瘤同时表达α和βA亚单位，这与血清抑制素-A水平升高有关。因此，虽然颗粒细胞肿瘤表达抑制素，但大多数上皮性肿瘤可能表达激活素，这一结果需要通过血清测量来证实。(C)1997年约翰惠氏父子有限公司。
Dimeric inhibin-A has been shown recently by a sensitive two-site ELISA assay to be a useful tumour marker in granulosa cell tumours of the ovary. It is also elevated in some patients with malignant epithelial ovarian tumours. To identify the precise subunits of inhibin expressed in ovarian tumours, three monoclonal antibodies, R1, E4, and INPRO (against the alpha C-, beta A-, and pro-alpha-subunits, respectively), were evaluated by immunohistochemistry on a panel of six granulosa cell and nine epithelial tumours. All granulosa cell tumours stained positively with E4 and R1, suggesting expression of dimeric inhibin-A; in two patients where serum levels were measured pre-operatively, they were elevated. The tumours also reacted weakly with INPRO, suggesting the presence of precursor forms of the alpha-subunit. Eight malignant epithelial ovarian tumours expressed the beta A-subunit only, as recognized by E4, while one tumour expressed both alpha- and beta A-subunits, which correlated with an elevated serum inhibin-A level. Thus, while granulosa cell tumours express inhibin, the majority of epithelial tumours probably express activin, a result which needs to be confirmed by serum measurements. (C) 1997 by John Whey & Sons, Ltd.