Increased sensitivity of malaria detection by nested polymerase chain reaction using simple sampling and DNA extraction
Increased sensitivity of malaria detection by nested polymerase chain reaction using simple sampling and DNA extraction
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DOI：
10.1016/s0020-7519(97)00147-1
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发表时间：
1997-12-01
影响因子：
4
通讯作者：
Singh, B
中科院分区：
文献类型：
--
作者：
Cox-Singh, J;Mahayet, S;Singh, B
疟疾仍然是世界上不发达和偏远地区的一种疾病。聚合酶链式反应(PCR)技术在疟疾流行病学中的应用有可能增加我们对这一疾病的认识和理解。为了在所有地理位置研究疟疾，重要的是保持用于聚合酶链式反应的样本采集和DNA提取的简单性。本文报道了一种从滤纸干血斑中提取DNA的方法，该方法能在不影响标本采集和DNA提取的简单性的情况下，对每亩L全血中的一种恶性疟原虫和两种间日疟原虫进行检测。(C)1997年澳大利亚寄生虫学学会。爱思唯尔科学有限公司出版。
Malaria remains a disease of underdeveloped and remote regions of the world. The application of polymerase chain reaction (PCR) technology to malaria epidemiology has the potential for increasing our knowledge and understanding of this disease. In order to study malaria in all geographical locations it is important that specimen collection and DNA extraction for PCR be kept simple. Here we report a method for extracting DNA from dried blood spots on filter paper which is capable of detecting one Plasmodium falciparum and two Plasmodium vivax parasites/mu l of whole blood by nested PCR without compromising the simplicity of specimen collection or DNA extraction. (C) 1997 Australian Society for Parasitology. Published by Elsevier Science Ltd.