Chinese Herbal Formulas Miao-Yi-Ai-Tang Inhibits the Proliferation and Migration of Lung Cancer Cells through Targeting β-Catenin/AXIN and Presents Synergistic Effect with Cisplatin Suppressing Lung Cancer
Chinese Herbal Formulas Miao-Yi-Ai-Tang Inhibits the Proliferation and Migration of Lung Cancer Cells through Targeting β-Catenin/AXIN and Presents Synergistic Effect with Cisplatin Suppressing Lung Cancer
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DOI：
10.1155/2020/2761850
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发表时间：
2020-01-20
影响因子：
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通讯作者：
Ge, Zhengxing
中科院分区：
文献类型：
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作者：
Li, Bo;Zhang, Wei;Ge, Zhengxing
Objective.肺癌是全球癌症死亡的主要原因之一，尽管筛查、预防和治疗方法有所改进，但五年生存率仍然很低。中草药已广泛用于肿瘤的预防和治疗。苗抑癌汤是一种新型中药制剂，具有潜在的抗癌作用。材料和方法。人小细胞肺癌细胞用于体外研究。分别用Miao和顺铂（DDP）处理后，用transwell法、伤口愈合法、CCK-8法和流式细胞术检测细胞的侵袭、迁移、增殖和凋亡。采用qRT-PCR和免疫组化方法检测β-catenin、AXIN和c-myc的表达。Western blotting检测β-catenin蛋白表达。建立肺癌NCI-H446皮下移植瘤模型，观察妙对肿瘤生长的影响。结果我们发现妙可抑制人肺癌细胞的侵袭、迁移和增殖，并促进其凋亡。同时，苗圩与顺铂对肺癌细胞的增殖和凋亡有协同调节作用。Miao能显著提高S期和G2期肺癌细胞的百分率。此外，Miao对c-myc、AXIN和beta-catenin的表达也有明显的抑制作用。Miao明显抑制体内肿瘤生长。结论.中药苗药可以通过靶向β-catenin/AXIN信号通路抑制肺癌。因此，我们的研究结果可能为肺癌的预防和治疗提供一种新的策略。
Objective. Lung cancer is one of the major causes of cancer deaths worldwide, and the five-year survival still remains low despite the improvement of screening, prevention, and treatment methods. Chinese herbal medicines have been widely used for tumor prevention and treatment. Miao-Yi-Ai-Tang (Miao) is a novel herbal formulation and shows a potential anticancer effect. Materials and Methods. Human Small Cell Lung Cancer Cell was used for study in vitro. After treatments by Miao and Cisplatin (DDP), the invasion, migration, proliferation, and apoptosis of cells were detected by transwell, wound healing, CCK-8, and flow cytometry, respectively. The expression of beta-catenin, AXIN, and c-myc was detected by qRT-PCR and immunohistochemistry staining. Western blotting was applied for measuring the protein expression of beta-catenin. Subcutaneously transplanted tumor model of lung cancer NCI-H446 was established to investigate the influence of Miao on tumor growth. Results. We found that Miao could inhibit invasion, migration, and proliferation and promote apoptosis of human lung cancer cells. Meanwhile, Miao and DDP presented synergy regulating the proliferation and apoptosis of lung cancer cells. The percentage of lung cancer cells in S and G2 stages was increased markedly by Miao. Besides, the expression of c-myc, AXIN, and beta-catenin was markedly inhibited by Miao. Tumor growth in vivo was markedly inhibited by Miao. Conclusions. Chinese herbal formulas Miao could suppress lung cancer through targeting the beta-catenin/AXIN signaling pathway. Therefore, our findings may provide a novel strategy for the prevention and treatment of lung cancer.