THE DEVELOPMENT OF MUCOSAL IMMUNITY IN GUINEA-PIG MIDDLE EARS
THE DEVELOPMENT OF MUCOSAL IMMUNITY IN GUINEA-PIG MIDDLE EARS
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DOI：
10.1016/0165-5876(80)90007-5
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发表时间：
1980-01-01
影响因子：
1.5
通讯作者：
WATANABE, N
中科院分区：
文献类型：
--
作者：
MOGI, G;MAEDA, S;WATANABE, N
最近的研究表明，中耳是一个能够对抗原物质做出免疫反应的器官，目前尚不清楚中耳何时以及如何开始提供免疫防御系统。用直接免疫荧光法观察了20只发育期豚鼠和5只正常成年豚鼠咽鼓管和鼓泡粘膜中免疫球蛋白[Ig]的形成细胞及分泌成分和乳铁蛋白等分泌蛋白。对鼻、喉、气管、小肠、脾、淋巴结等脏器进行了观察。本文观察了6只发育期豚鼠和39只成年豚鼠直接鼓室抗原攻击后中耳粘膜的变化。出生后第7天，输卵管粘膜内开始出现IgA和IgM形成细胞，而发育中的豚鼠则几乎不可能见到IgG1和IgG2形成细胞。抗原刺激后，中耳粘膜各类型免疫球蛋白形成细胞增多。显然，IgA等类的局部合成潜伏于中耳，幼年动物的中耳易受抗原刺激，发育中的动物中耳具有潜在的免疫反应性。
Recent studies suggested that the middle ear is an organ capable of responding immunologically to antigenic substance; it is still not known when and how the middle ear starts to provide the immunologic defense system. Immunoglobulin [Ig] forming cells of different classes and secretory proteins, such as secretory component and lactoferrin, in the mucous membrane of eustachian tube and tympanic bulla of 20 developing and 5 normal adult guinea pigs were investigated by the use of a direct immunofluorescence technique. Other organs, such as nose, larynx, trachea, small intestine, spleen and lymph node were investigated. Changes in the middle ear mucosa were observed after antigenic challenges directly to the tympanic cavity of 6 developing and 39 adult guinea pigs. IgA and IgM forming cells began to appear in the tubal mucosa on 7th postnatal day; it was scarcely possible to find IgG1 and IgG2 forming cells in developing guinea pigs. Ig forming cells of all classes increased in the middle ear mucosa after the antigenic stimuli. Apparently, local synthesis of IgA and other classes is latent in the middle ear, the middle ear of immature animals is vulnerable to antigenic stimuli, and the middle ear of developing animals possesses potential immune responsiveness.