Glomerular size in renal dysplasia secondary to obstructive uropathy: a further exploration of the fetal lamb model.
Glomerular size in renal dysplasia secondary to obstructive uropathy: a further exploration of the fetal lamb model.
复制标题
继发于梗阻性尿病的肾发育不良的肾小球大小：胎儿羔羊模型的进一步探索。
DOI：
10.1053/jpsu.2000.18344
复制
发表时间：
2000
影响因子：
2.4
通讯作者：
Y. Seki
中科院分区：
文献类型：
--
作者：
H. Kitagawa;K. Pringle;J. Zuccollo;J. Koike;K. Nakada;M. Ikoma;Y. Seki
背景/目的
在肾小球发生早期形成梗阻性尿路病变会产生多囊性发育不良肾（MCDK）。测量肾小球平均平面面积 (GMPA) 可以阐明 MCDK 的发病机制。
方法
孕60天的胎羔结扎左侧输尿管，孕145天剖腹产。记录肾脏重量和长度。使用计算机化面积计测量切片肾脏的 3 个区域（外、中、内）的 GMPA。将梗阻的肾脏与对侧未梗阻的肾脏进行比较。使用未配对的学生 t 检验来确定显着性。
结果
一只母羊流产了。 5 只 60 天的羔羊中有 4 只（80%）存活下来。所有人都有发育不良的肾脏。 MCDK 中的平均肾脏重量为 4.3 +/- 0.84 g，对照组为 16.8 +/- 3.6 g（P< .05）。 MCDK外区、中区和内区的GMPA分别为2.7×10(-3)mm2、3.2×10(-3)mm2和4.0×10(-3)mm2。对照尺寸为 2.8 x 10(-3) mm2、4.4 x 10(-3) mm2 和 6.0 x 10(-3) mm2。 60天胎儿肾脏的肾小球分别为3.0×10(-3)mm2、6.1×10(-3)mm2和11.0×10(-3)mm2。 MCDK 的内部和中部区域的肾小球比对照组更小。
结论
胎儿肾小球似乎是从肾脏的内部区域生长的。早期尿路梗阻会阻止这种生长。
BACKGROUND/PURPOSE
Creating an obstructive uropathy early in glomerulogenesis would produce multicystic dysplastic kidneys (MCDK). Measuring the mean planar area of the glomeruli (GMPA) may clarify the pathogenesis of MCDK.
METHODS
Fetal lambs at 60 days' gestation had their left ureter ligated and were delivered by cesarian section at 145 days' gestation. Kidney weight and length were recorded. GMPA in 3 zones (outer, middle, inner) of the sectioned kidney was measured using a computerized planimeter. The obstructed kidneys were compared with contralateral unobstructed kidneys. The unpaired Student's t test was used to determine significance.
RESULTS
One ewe miscarried. Four of 5 (80%) 60-day lambs survived. All had dysplastic kidneys. Mean kidney weights were 4.3 +/- 0.84 g in MCDK and 16.8 +/- 3.6 g in controls (P< .05). The GMPA of the outer, middle, and inner zones of the MCDK were 2.7 x 10(-3) mm2, 3.2 x 10(-3) mm2, and 4.0 x 10(-3) mm2, respectively. Controls were 2.8 x 10(-3) mm2, 4.4 x 10(-3) mm2, and 6.0 x 10(-3) mm2. The glomeruli of 60-day fetal kidneys were 3.0 x 10(-3) mm2, 6.1 x 10(-3) mm2, and 11.0 x 10(-3) mm2. MCDK had smaller glomeruli in the inner and middle zones than controls.
CONCLUSION
Fetal glomeruli appear to grow from the inner zone of the kidney. Early urinary tract obstruction stops this growth.