Transcriptional profiling of Dictyostelium with RNA sequencing.
Transcriptional profiling of Dictyostelium with RNA sequencing.
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DOI：
10.1007/978-1-62703-302-2_8
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发表时间：
2013-01-01
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影响因子：
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通讯作者：
Zupan, Blaz
中科院分区：
文献类型：
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作者：
Miranda, Edward Roshan;Rot, Gregor;Zupan, Blaz
转录分析方法已用于分析盘基网柄菌的各种生物过程。高通量测序的最新进展提高了转录分析的分辨率和动态范围。在这里，我们描述了使用 Illumina 技术进行 RNA 测序来生成转录谱的用途。我们还描述了数据映射和存储的方法，以及在线和离线数据分析的常用和专用工具。
Transcriptional profiling methods have been utilized in the analysis of various biological processes in Dictyostelium. Recent advances in high-throughput sequencing have increased the resolution and the dynamic range of transcriptional profiling. Here we describe the utility of RNA sequencing with the Illumina technology for production of transcriptional profiles. We also describe methods for data mapping and storage as well as common and specialized tools for data analysis, both online and offline.